PMID- 22160624
OWN - NLM
STAT- MEDLINE
DCOM- 20120419
LR  - 20120302
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 157
IP  - 3
DP  - 2012 Mar
TI  - HIV-infection resistance in PMBC-derived dendritic cells modified with 
      recombinant virus.
PG  - 413-21
LID - 10.1007/s00705-011-1185-7 [doi]
AB  - This study aimed to identify the characteristics of 
      recombinant-adenovirus-modified PBMC-derived dendritic cells and their resistance 
      to HIV-1 infection by integrating the CCR5∆32, CCR5siRNA, HIV-1 pol and HIV-1 int 
      genes into a recombinant adenovirus vector using the AdEasy system. Dendritic 
      cells (DCs) were isolated from human PBMCs from blood of healthy donors. The 
      expression of CCR5∆32, CCR5, CXCR4 and HIV-1 p24 in PBMCs or modified cells was 
      measured by western blot, p24 expression in cell lysates was measured by ELISA, 
      and HIV-1 entry was measured by beta-galactosidase assay. Furthermore, T-cell 
      immunity induced by the recombinant adenovirus was measured by ELISPOT assay. 
      After the cells were modified by Ad-R5∆32siRNA, the expression of CCR5∆32 
      increased, while the expression of CCR5 and CXCR4 decreased. There was no adverse 
      effect of adenoviral gene transfer on DC development. CD83 expression on the 
      surface of mature DCs did not change after gene transfer. The expression of p24 
      remained at low levels in modified cells when challenged by HIV-1. The modified 
      cells showed resistance to HIV-1 infection. Results indicated that 
      recombinant-adenovirus-modified cells demonstrated good resistance to HIV-1 
      infection. Modification of HSC-derived immune cells, such as DCs, may be a potent 
      strategy to resist HIV-1 infection.
FAU - Xia, Cheng-Lai
AU  - Xia CL
AD  - Department of Pharmacy, The Third Affiliated Hospital, Medical University of 
      Guangzhou, Guangzhou 510150, China.
FAU - Zhu, Ping
AU  - Zhu P
FAU - Cai, Yan-Tao
AU  - Cai YT
FAU - Zhu, Guang-Bin
AU  - Zhu GB
FAU - Mei, Zheng-Rong
AU  - Mei ZR
FAU - Huang, Hanhui
AU  - Huang H
FAU - Luo, Di-Xian
AU  - Luo DX
FAU - Yan, Peng-Ke
AU  - Yan PK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111211
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Receptors, CCR5)
RN  - 0 (Receptors, HIV)
RN  - 0 (Recombinant Proteins)
RN  - 0 (pol Gene Products, Human Immunodeficiency Virus)
RN  - EC 2.7.7.- (HIV Integrase)
SB  - IM
MH  - Adenoviridae/*genetics
MH  - Dendritic Cells/*virology
MH  - Gene Silencing
MH  - *Genetic Vectors
MH  - HIV Integrase/biosynthesis/genetics
MH  - HIV-1/*pathogenicity
MH  - Humans
MH  - Receptors, CCR5/biosynthesis/genetics
MH  - Receptors, HIV/biosynthesis/genetics
MH  - Recombinant Proteins/biosynthesis/genetics
MH  - *Virus Attachment
MH  - *Virus Replication
MH  - pol Gene Products, Human Immunodeficiency Virus/biosynthesis/genetics
EDAT- 2011/12/14 06:00
MHDA- 2012/04/20 06:00
CRDT- 2011/12/14 06:00
PHST- 2011/08/25 00:00 [received]
PHST- 2011/11/29 00:00 [accepted]
PHST- 2011/12/14 06:00 [entrez]
PHST- 2011/12/14 06:00 [pubmed]
PHST- 2012/04/20 06:00 [medline]
AID - 10.1007/s00705-011-1185-7 [doi]
PST - ppublish
SO  - Arch Virol. 2012 Mar;157(3):413-21. doi: 10.1007/s00705-011-1185-7. Epub 2011 Dec 
      11.