PMID- 22161099
OWN - NLM
STAT- MEDLINE
DCOM- 20120412
LR  - 20111214
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 28
IP  - 6
DP  - 2011 Dec
TI  - [Optimized procedure for fluorescence in situ hybridization in rapid prenatal 
      diagnosis of common aneuploidy].
PG  - 658-60
LID - 10.3760/cma.j.issn.1003-9406.2011.06.013 [doi]
AB  - OBJECTIVE: To optimize the procedure of fluorescence in situ hybridization 
      (FISH), and evaluate it in rapid prenatal diagnosis of common aneuploidy. 
      METHODS: Amniotic fluid samples from 300 pregnant women were tested by both 
      interphase FISH and conventional cell culture for karyotyping from September 2009 
      and September 2010. RESULTS: Seven cases of trisomy 21, 4 of trisomy 18, 2 of 
      monosomy X, 1 of XXY, 1 of XXX, and 1 of triploidy were detected by FISH in the 
      300 amniotic fluid samples. It was concordant with the results from conventional 
      karyotype analysis. The concordance rate was 100%. CONCLUSION: Through a 
      technical modification of FISH procedure, the detection accuracy and specificity 
      was not affected but testing cost reduced greatly. It can be used in rapid 
      prenatal diagnosis of common aneuploidy.
FAU - Wu, Jing
AU  - Wu J
AD  - Department of Obstetrics and Gynecology, Southern Medical University, Guangzhou, 
      People's Republic of China.
FAU - Zhong, Mei
AU  - Zhong M
FAU - Lu, Jian
AU  - Lu J
FAU - Pan, Xiao-ying
AU  - Pan XY
FAU - Guo, Li
AU  - Guo L
FAU - Wang, Ting
AU  - Wang T
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Adult
MH  - Amniotic Fluid/cytology
MH  - *Aneuploidy
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
MH  - Young Adult
EDAT- 2011/12/14 06:00
MHDA- 2012/04/13 06:00
CRDT- 2011/12/14 06:00
PHST- 2011/12/14 06:00 [entrez]
PHST- 2011/12/14 06:00 [pubmed]
PHST- 2012/04/13 06:00 [medline]
AID - 940628132 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2011.06.013 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2011 Dec;28(6):658-60. doi: 
      10.3760/cma.j.issn.1003-9406.2011.06.013.