PMID- 22161103
OWN - NLM
STAT- MEDLINE
DCOM- 20120412
LR  - 20111214
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 28
IP  - 6
DP  - 2011 Dec
TI  - [Analysis of sex chromosome mosaicisms in early cleavage-stage human embryos and 
      blastocysts with poor embryo quality scores].
PG  - 675-8
LID - 10.3760/cma.j.issn.1003-9406.2011.06.017 [doi]
AB  - OBJECTIVE: To analyze sex chromosome mosaicisms in early cleavage-stage human 
      embryos and blastocysts with poor embryo quality score based on the numbers of 
      pronucleus(PN) zygotes using X,Y dual color fluorescence in situ hybridization 
      (FISH), and to discuss the possible mechanisms. METHODS: Fresh or frozen-thawed 
      early cleavage-stage human embryos and blastocysts with poor embryo quality score 
      not suitable for embryo transfer were studied with dual color FISH. RESULTS: 
      Double signal rate of 2PN among early cleavage-stage embryos was 66.67%, which 
      was significantly higher than 1PN and 3PN embryos. Single signal rate of 1PN 
      early cleavage-stage embryos was 90.41%, which was significantly higher than 2PN 
      and 3PN ones. Three signal rate of 3PN early cleavage-stage embryos was 28.00%, 
      which was significantly higher than 1PN and 2PN ones. Double signal rate of 3PN 
      ones was 46.00%, which was significantly higher than 1PN ones. The polyploid rate 
      of frozen-thawed early cleavage-stage embryos was 23.53%, which was slightly 
      higher than that of fresh embryos, but with no statistical significance. The 
      mosaicism rate of 24 blastocysts was 100.00% and the double signal dominant (>/= 
      50%) rate was 62.50%, which was significantly higher than the rate of early 
      cleavage-stage embryos. CONCLUSION: Using 2PN as the criterion for embryo quality 
      score cannot guarantee the selection of normal fertilized embryo for 
      transplantation. Frozen-thawed embryos may harbor more polyploid cells. To avoid 
      the selection of embryos with abnormal chromosomes, combinations of 
      pre-implantation genetic diagnosis (PGD) and prenatal diagnosis are necessary. 
      Meanwhile, blastocysts with poor quality scores may provide an important source 
      for embryo stem cells.
FAU - Ou, Jian
AU  - Ou J
AD  - Suzhou Municipal Hospital, Suzhou, Jiangsu, People's Republic of China.
FAU - Wang, Wei
AU  - Wang W
FAU - Ding, Jie
AU  - Ding J
FAU - Gu, Bin
AU  - Gu B
FAU - Zheng, Ai-yan
AU  - Zheng AY
FAU - Wang, Fu-xin
AU  - Wang FX
FAU - Li, Hong
AU  - Li H
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Blastocyst/*metabolism
MH  - Cleavage Stage, Ovum/*metabolism
MH  - Fertilization in Vitro
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mosaicism/*embryology
MH  - *Sex Chromosomes
EDAT- 2011/12/14 06:00
MHDA- 2012/04/13 06:00
CRDT- 2011/12/14 06:00
PHST- 2011/12/14 06:00 [entrez]
PHST- 2011/12/14 06:00 [pubmed]
PHST- 2012/04/13 06:00 [medline]
AID - 940628136 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2011.06.017 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2011 Dec;28(6):675-8. doi: 
      10.3760/cma.j.issn.1003-9406.2011.06.017.