PMID- 22167078
OWN - NLM
STAT- MEDLINE
DCOM- 20121203
LR  - 20211021
IS  - 1432-184X (Electronic)
IS  - 0095-3628 (Linking)
VI  - 63
IP  - 4
DP  - 2012 May
TI  - Acidobacteria in freshwater ponds at Donana National Park, Spain.
PG  - 844-55
LID - 10.1007/s00248-011-9988-3 [doi]
AB  - The Acidobacteria show a widespread distribution in natural ecosystems. In this 
      study, we analyzed the presence of Acidobacteria in freshwater ponds at Donana 
      National Park (southwestern Spain). Nucleic acid sequence analysis, quantitative, 
      real-time RT-PCR, and fluorescence in situ hybridization (FISH) were carried out. 
      Acidobacteria in these aquatic environments were investigated using their 16S and 
      23S rDNA sequences and acidobacterial specific primer pairs through phylogenetic 
      approaches. The presence of up to five subdivisions of Acidobacteria was detected 
      during this study. The analyzed ponds exhibited distinctive patterns of 
      acidobacterial clades. In order to detect their role in ecosystem functions, 
      metabolically active Acidobacteria were detected based upon rRNA analyses. 
      Quantitative, real-time RT-PCR showed a low percentage of metabolically active 
      Acidobacteria at suboxic zones within the water column covered by surface Fe-rich 
      films. Oxygen-saturated areas showed around 4% of total bacterial RNA belonging 
      to Acidobacteria both in the water column and the sediment surface. The 
      morphology of the most abundant Acidobacteria was revealed by FISH as cocci 
      generally in pairs or chains. Enrichment cultures were also obtained and 
      indicated a putative metabolism based on aerobic and heterotrophic 
      characteristics likely taking advantage of the abundant organic matter present at 
      the investigated sites. These results represent a significant contribution toward 
      understanding the distribution and ecological role of the phylum Acidobacteria in 
      natural ecosystems, specifically at Donana National Park freshwater ponds.
FAU - Zimmermann, Johannes
AU  - Zimmermann J
AD  - Instituto de Recursos Naturales y Agrobiologia, CSIC, Avda. Reina Mercedes 10, 
      Seville, Spain.
FAU - Portillo, M Carmen
AU  - Portillo MC
FAU - Serrano, Laura
AU  - Serrano L
FAU - Ludwig, Wolfgang
AU  - Ludwig W
FAU - Gonzalez, Juan M
AU  - Gonzalez JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111215
PL  - United States
TA  - Microb Ecol
JT  - Microbial ecology
JID - 7500663
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (RNA, Ribosomal, 23S)
SB  - IM
MH  - Acidobacteria/classification/*genetics/growth & development/isolation & 
      purification
MH  - DNA Primers
MH  - Ecosystem
MH  - In Situ Hybridization, Fluorescence
MH  - Phylogeny
MH  - Ponds/*microbiology
MH  - RNA, Ribosomal, 16S/genetics
MH  - RNA, Ribosomal, 23S/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Spain
EDAT- 2011/12/15 06:00
MHDA- 2012/12/10 06:00
CRDT- 2011/12/15 06:00
PHST- 2011/09/06 00:00 [received]
PHST- 2011/11/23 00:00 [accepted]
PHST- 2011/12/15 06:00 [entrez]
PHST- 2011/12/15 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - 10.1007/s00248-011-9988-3 [doi]
PST - ppublish
SO  - Microb Ecol. 2012 May;63(4):844-55. doi: 10.1007/s00248-011-9988-3. Epub 2011 Dec 
      15.