PMID- 22172733
OWN - NLM
STAT- MEDLINE
DCOM- 20120510
LR  - 20161126
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1817
IP  - 4
DP  - 2012 Apr
TI  - Resonance Raman applications in investigations of cytochrome c oxidase.
PG  - 575-8
LID - 10.1016/j.bbabio.2011.11.016 [doi]
AB  - Recent applications of resonance Raman (RR) spectroscopy in investigations of 
      cytochrome c oxidase (CcO) are reviewed. Red-excited RR spectra for the fully 
      oxidized "as-isolated" CcO tuned to the ligand-to-metal charge transfer 
      absorption band at 655nm exhibit a Raman band at 755cm(-1) assignable to the 
      nu(OO) stretching mode of a peroxide. Binding of CN(-) diminishes the RR band 
      concomitant with the loss of the charge transfer absorption band. This suggests 
      that a peroxide forms a bridge between heme a(3) and Cu(B). Time-resolved RR 
      spectroscopy of whole mitochondria identified a band at 571cm(-1) arising from 
      the oxygenated intermediate at Deltat=0.4, 0.6 and 1.4ms. Bands at 804 and 780cm(-1) 
      of the P and F intermediates were observed at Deltat=0.6 and 1.4ms, respectively. The 
      coordination geometries of the three intermediates are essentially the same as 
      the respective species observed for solubilized CcO. However, the lifetime of the 
      oxygenated intermediate in mitochondria was significantly longer than the 
      lifetime of this intermediate determined for solubilized CcO. This phenomenon is 
      due either to the pH effect of mitochondrial matrix, the effect of DeltapH and/or DeltaPsi 
      across the membrane, or the effect of interactions with other membrane components 
      and/or phospholipids.
CI  - Copyright A(c) 2011 Elsevier B.V. All rights reserved.
FAU - Ogura, Takashi
AU  - Ogura T
AD  - University of Hyogo, Hyogo, Japan. ogura@sci.u-hyogo.ac.jp
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20111208
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 42VZT0U6YR (Heme)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Electron Transport
MH  - Electron Transport Complex IV/*metabolism
MH  - Heme/metabolism
MH  - Kinetics
MH  - Mitochondria/*metabolism
MH  - Oxidation-Reduction
MH  - Oxygen/*metabolism
MH  - Spectrum Analysis, Raman/*methods
EDAT- 2011/12/17 06:00
MHDA- 2012/05/11 06:00
CRDT- 2011/12/17 06:00
PHST- 2011/09/22 00:00 [received]
PHST- 2011/11/24 00:00 [revised]
PHST- 2011/11/24 00:00 [accepted]
PHST- 2011/12/17 06:00 [entrez]
PHST- 2011/12/17 06:00 [pubmed]
PHST- 2012/05/11 06:00 [medline]
AID - S0005-2728(11)00288-X [pii]
AID - 10.1016/j.bbabio.2011.11.016 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2012 Apr;1817(4):575-8. doi: 10.1016/j.bbabio.2011.11.016. 
      Epub 2011 Dec 8.