PMID- 22172872
OWN - NLM
STAT- MEDLINE
DCOM- 20120329
LR  - 20111216
IS  - 1873-2623 (Electronic)
IS  - 0041-1345 (Linking)
VI  - 43
IP  - 10
DP  - 2011 Dec
TI  - High expression of Fas ligand on cord blood dendritic cells: a possible 
      immunoregulatory mechanism after cord blood transplantation.
PG  - 3913-9
LID - 10.1016/j.transproceed.2011.10.040 [doi]
AB  - BACKGROUND: Allogeneic cord blood transplantation is associated with less severe 
      graft-versus-host disease (GVHD). Dendritic cells (DCs), as the most potent 
      antigen-presenting cells of the immune system, play a central role in the 
      development of GVHD. Because apoptosis induction is one of the known mechanisms 
      that DCs use to regulate T-cell responses, we studied the immunostimulatory and 
      apoptosis induction capacities of cord blood dendritic cells (CBDCs) and 
      peripheral blood dendritic cells (PBDCs) to evaluate the mechanisms underlying 
      the lower incidence of GVHD after cord blood transplantation. Presence of 
      apoptosis-related markers Fas, Fas ligand (FasL), and CD40 and costimulatory 
      molecules, along with the proportion of myeloid and lymphoid DCs subsets, were 
      also measured on CBDCs and PBDCs. METHODS: Fresh CBDCs and PBDCs were isolated 
      from cord and peripheral mononuclear cells as lineage-negative cells by using 
      monoclonal antibodies against CD3, CD11b, CD14, CD16, CD19, CD56, CD34, and 
      CD66b. DCs were cocultured with allogeneic T cells, and the effect of CBDCs and 
      PBDCs on T-cell apoptosis and proliferation were determined through flow 
      cytometric analysis and 3H-thymidine incorporation. RESULTS: Our findings showed 
      that CBDCs markedly augment apoptosis of CD3+ T-cells. FasL expression on CBDCs 
      was significantly higher than on PBDCs. However, there was no difference between 
      Fas expression on CBDCs and PBDCs. Moreover, CBDCs were poor stimulators of 
      allogenic T cells in mixed leukocyte reaction compared with adult peripheral 
      blood DCs. They also displayed decreased expression of HLA-DR and CD86 molecules. 
      The ratio of lymphoid DCs (CD11c-, CD123+) to myeloid DCs (CD11c+, CD123-) was 
      also significantly higher in CBDCs compared with PBDCs. CONCLUSIONS: It seems 
      that less severe GVHD after cord blood transplantation is due not only to a 
      higher degree of immaturity of CBDCs, but also to delivery of apoptotic signals 
      to the host T cells that recognize allo-MHC molecules on CBDCs in the early phase 
      of immune response.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Naderi, N
AU  - Naderi N
AD  - Immunology Department, Hormozgan University of Medical Sciences, Bandarabbas, 
      Hormozgan, Iran.
FAU - Moazzeni, S M
AU  - Moazzeni SM
FAU - Pourfathollah, A A
AU  - Pourfathollah AA
FAU - Alimoghaddam, K
AU  - Alimoghaddam K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (FASLG protein, human)
RN  - 0 (Fas Ligand Protein)
SB  - IM
MH  - Apoptosis
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Cord Blood Stem Cell Transplantation/*adverse effects
MH  - Dendritic Cells/immunology/*transplantation
MH  - Fas Ligand Protein/blood/*metabolism
MH  - Fetal Blood/*cytology
MH  - Flow Cytometry
MH  - Graft vs Host Disease/*immunology/pathology/prevention & control
MH  - Humans
MH  - Immunophenotyping
MH  - Lymphocyte Culture Test, Mixed
MH  - T-Lymphocytes/*immunology/pathology
EDAT- 2011/12/17 06:00
MHDA- 2012/03/30 06:00
CRDT- 2011/12/17 06:00
PHST- 2011/10/04 00:00 [received]
PHST- 2011/10/07 00:00 [accepted]
PHST- 2011/12/17 06:00 [entrez]
PHST- 2011/12/17 06:00 [pubmed]
PHST- 2012/03/30 06:00 [medline]
AID - S0041-1345(11)01423-0 [pii]
AID - 10.1016/j.transproceed.2011.10.040 [doi]
PST - ppublish
SO  - Transplant Proc. 2011 Dec;43(10):3913-9. doi: 10.1016/j.transproceed.2011.10.040.