PMID- 22173307
OWN - NLM
STAT- MEDLINE
DCOM- 20120405
LR  - 20211021
IS  - 1869-1889 (Electronic)
IS  - 1674-7305 (Print)
IS  - 1674-7305 (Linking)
VI  - 54
IP  - 11
DP  - 2011 Nov
TI  - Cellular membrane cholesterol is required for porcine reproductive and 
      respiratory syndrome virus entry and release in MARC-145 cells.
PG  - 1011-8
LID - 10.1007/s11427-011-4236-0 [doi]
AB  - Cholesterol represents one of the key constituents of small, dynamic, sterol- and 
      sphingolipid-enriched domains on the plasma membrane. It has been reported that 
      many viruses depend on plasma membrane cholesterol for efficient infection. In 
      this study, the role of the plasma membrane cholesterol in porcine reproductive 
      and respiratory syndrome virus (PRRSV) infection of MARC-145 cells was 
      investigated. Pretreatment of MARC-145 cells with methyl-beta-cyclodextrin (MbetaCD), a 
      drug used to deplete cholesterol from cellular membrane, significantly reduced 
      PRRSV infection in a dose-dependent manner. This inhibition was partially 
      reversed by supplementing exogenous cholesterol following MbetaCD treatment, 
      suggesting that the inhibition of PRRSV infection was specifically mediated by 
      removal of cellular cholesterol. Further detailed studies showed that depletion 
      of cellular membrane cholesterol significantly inhibited virus entry, especially 
      virus attachment and release. These results indicate that the presence of 
      cholesterol in the cellular membrane is a key component of PRRSV infection.
FAU - Sun, Ying
AU  - Sun Y
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University, Wuhan 430070, China.
FAU - Xiao, ShaoBo
AU  - Xiao S
FAU - Wang, Dang
AU  - Wang D
FAU - Luo, Rui
AU  - Luo R
FAU - Li, Bin
AU  - Li B
FAU - Chen, HuanChun
AU  - Chen H
FAU - Fang, LiuRong
AU  - Fang L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111216
PL  - China
TA  - Sci China Life Sci
JT  - Science China. Life sciences
JID - 101529880
RN  - 0 (beta-Cyclodextrins)
RN  - 0 (methyl-beta-cyclodextrin)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Membrane/*chemistry/drug effects/metabolism
MH  - Cell Survival
MH  - Cholesterol/*metabolism
MH  - Porcine Reproductive and Respiratory Syndrome/metabolism
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/*metabolism/pathogenicity
MH  - Swine
MH  - *Virus Internalization
MH  - *Virus Release
MH  - beta-Cyclodextrins/pharmacology
PMC - PMC7088586
EDAT- 2011/12/17 06:00
MHDA- 2012/04/06 06:00
PMCR- 2011/12/16
CRDT- 2011/12/17 06:00
PHST- 2010/12/10 00:00 [received]
PHST- 2011/05/27 00:00 [accepted]
PHST- 2011/12/17 06:00 [entrez]
PHST- 2011/12/17 06:00 [pubmed]
PHST- 2012/04/06 06:00 [medline]
PHST- 2011/12/16 00:00 [pmc-release]
AID - 4236 [pii]
AID - 10.1007/s11427-011-4236-0 [doi]
PST - ppublish
SO  - Sci China Life Sci. 2011 Nov;54(11):1011-8. doi: 10.1007/s11427-011-4236-0. Epub 
      2011 Dec 16.