PMID- 22175881
OWN - NLM
STAT- MEDLINE
DCOM- 20120308
LR  - 20191210
IS  - 1532-4117 (Electronic)
IS  - 1093-4529 (Linking)
VI  - 46
IP  - 14
DP  - 2011
TI  - Propidium iodide staining method for testing the cytotoxicity of 
      2,4,6-trichlorophenol and perfluorooctane sulfonate at low concentrations with 
      Vero cells.
PG  - 1769-75
LID - 10.1080/10934529.2011.624016 [doi]
AB  - In this article, the feasibility of propidium iodide (PI) staining based on cell 
      membrane damage as a sensitive and quantitative method to test the cytotoxicity 
      of typical lipophilic compounds including 2,4,6-trichlorophenol (TCP) and 
      perfluorooctane sulfonate (PFOS) was examined. The sensitivity of PI staining was 
      compared to that of the methylthiazol-2-yl-2,5-diphenyl tetrazolium bromide (MTT) 
      assay. We found a good correlation between PI uptake and increasing 
      concentrations of TCP (10-50 muM) and PFOS (20-50 muM) at lower concentrations than 
      those between growth inhibition ratio determined by the MTT assay and increasing 
      concentrations of TCP (150-400 muM) and PFOS (100-500 muM). These findings 
      indicated that the PI staining was more sensitive than the MTT assay. 
      Furthermore, both the amount of PI uptake and the ratio of morphologically 
      changed cells increased with increasing TCP concentrations, suggesting that PI 
      staining may be a suitable quantitative substitute for the original method based 
      on the observation of morphological changes in Vero cells. Our data also suggest 
      that it is possible that the cell membrane damage that resulted in increased PI 
      uptake may be due to variations in the phospholipid and protein content of the 
      membrane, which are affected by interactions between the lipophilic compounds and 
      components of the cell membrane.
FAU - Liao, Ting T
AU  - Liao TT
AD  - College of Environmental Science and Engineering, State Key Laboratory of 
      Pollution Control and Resource Reuse, Tongji University, Shanghai, China.
FAU - Jia, Ru W
AU  - Jia RW
FAU - Shi, Yan L
AU  - Shi YL
FAU - Jia, Jian W
AU  - Jia JW
FAU - Wang, Lei
AU  - Wang L
FAU - Chua, Hong
AU  - Chua H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Environ Sci Health A Tox Hazard Subst Environ Eng
JT  - Journal of environmental science and health. Part A, Toxic/hazardous substances & 
      environmental engineering
JID - 9812551
RN  - 0 (Alkanesulfonic Acids)
RN  - 0 (Chlorophenols)
RN  - 0 (Coloring Agents)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Fluorocarbons)
RN  - 0 (Membrane Proteins)
RN  - 0 (Phospholipids)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - 36015-30-2 (Propidium)
RN  - 9H2MAI21CL (perfluorooctane sulfonic acid)
RN  - EUY85H477I (thiazolyl blue)
RN  - MHS8C5BAUZ (2,4,6-trichlorophenol)
SB  - IM
MH  - Alkanesulfonic Acids/*toxicity
MH  - Animals
MH  - Cell Membrane/*drug effects/metabolism
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Chlorocebus aethiops
MH  - Chlorophenols/*toxicity
MH  - *Coloring Agents
MH  - Environmental Pollutants/toxicity
MH  - Fluorocarbons/*toxicity
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Membrane Proteins/metabolism
MH  - Phospholipids/metabolism
MH  - *Propidium
MH  - Tetrazolium Salts
MH  - Thiazoles
MH  - Vero Cells
EDAT- 2011/12/20 06:00
MHDA- 2012/03/09 06:00
CRDT- 2011/12/20 06:00
PHST- 2011/12/20 06:00 [entrez]
PHST- 2011/12/20 06:00 [pubmed]
PHST- 2012/03/09 06:00 [medline]
AID - 10.1080/10934529.2011.624016 [doi]
PST - ppublish
SO  - J Environ Sci Health A Tox Hazard Subst Environ Eng. 2011;46(14):1769-75. doi: 
      10.1080/10934529.2011.624016.