PMID- 22176213
OWN - NLM
STAT- MEDLINE
DCOM- 20120725
LR  - 20211203
IS  - 1557-7430 (Electronic)
IS  - 1044-5498 (Print)
IS  - 1044-5498 (Linking)
VI  - 31
IP  - 5
DP  - 2012 May
TI  - Molecular characterization and functional analysis of Cashmere goat mammalian 
      target of rapamycin.
PG  - 839-44
LID - 10.1089/dna.2011.1393 [doi]
AB  - The mammalian target of rapamycin (mTOR) is an evolutionarily conserved protein 
      kinase that belongs to the phosphatidylinositol kinase-related kinase family. We 
      describe our molecular characterization of mTOR and its function (GenBank 
      accession HM114224) in Cashmere goat (Capra hircus). The goat mTOR complementary 
      DNA is 8617 bp, comprising an open reading frame of 7650 bp--corresponding to a 
      polypeptide of 2549 amino acids--and a 909 bp 3' untranslated region with a polyA 
      tract and a polyadenylation signal at nucleotides 8575-8580. In a bioinformatics 
      analysis, goat mTOR has typical sites of activity and domains. mTOR mRNA was 
      measured in brain, heart, testis, liver, spleen, kidney, and lung by real-time 
      polymerase chain reaction, and the expression of mTOR in fetal fibroblasts was 
      detected by western blot. The viability of fetal fibroblasts was inhibited on 
      treatment with CCI-779, a specific inhibitor of mTOR. Our data supplied evidence 
      that the transcription of mTOR was detected in the seven tissues in Cashmere 
      goat, and mTOR protein was translated in fetal fibroblasts. The proliferation of 
      fetal fibroblasts decreases on inhibition of mTOR.
FAU - Liang, Yan
AU  - Liang Y
AD  - College of Life Science, Inner Mongolia University , The Key Laboratory of Mammal 
      Reproductive Biology and Biotechnology, Ministry of Education, Hohhot, People's 
      Republic of China .
FAU - Bao, Wen-Lei
AU  - Bao WL
FAU - Bao, Chan-Chan
AU  - Bao CC
FAU - Miao, Xiu-Fen
AU  - Miao XF
FAU - Hao, Hui-Fang
AU  - Hao HF
FAU - Li, Shu-Yu
AU  - Li SY
FAU - Wang, Zhi-Gang
AU  - Wang ZG
FAU - Liu, Dong-Jun
AU  - Liu DJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111216
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 624KN6GM2T (temsirolimus)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Western
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Fetus/cytology/drug effects/*metabolism
MH  - Fibroblasts/cytology/drug effects/*metabolism
MH  - Goats
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Protein Conformation
MH  - Protein Kinase Inhibitors/pharmacology
MH  - RNA, Messenger/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Sirolimus/analogs & derivatives/pharmacology
MH  - TOR Serine-Threonine Kinases/antagonists & inhibitors/*genetics/*metabolism
MH  - Tissue Distribution
PMC - PMC3358123
EDAT- 2011/12/20 06:00
MHDA- 2012/07/26 06:00
PMCR- 2013/05/01
CRDT- 2011/12/20 06:00
PHST- 2011/12/20 06:00 [entrez]
PHST- 2011/12/20 06:00 [pubmed]
PHST- 2012/07/26 06:00 [medline]
PHST- 2013/05/01 00:00 [pmc-release]
AID - 10.1089/dna.2011.1393 [pii]
AID - 10.1089/dna.2011.1393 [doi]
PST - ppublish
SO  - DNA Cell Biol. 2012 May;31(5):839-44. doi: 10.1089/dna.2011.1393. Epub 2011 Dec 
      16.