PMID- 22176341
OWN - NLM
STAT- MEDLINE
DCOM- 20120622
LR  - 20181201
IS  - 1472-765X (Electronic)
IS  - 0266-8254 (Linking)
VI  - 54
IP  - 3
DP  - 2012 Mar
TI  - Dimethyl formamide-free, urea-NaCl fluorescence in situ hybridization assay for 
      Staphylococcus aureus.
PG  - 263-6
LID - 10.1111/j.1472-765X.2011.03197.x [doi]
AB  - AIMS: To test the feasibility of identifying Staphylococcus aureus with a 
      fluorescence in situ hybridization (FISH) assay that uses a single hot-plate and 
      urea-NaCl reagents. METHODS AND RESULTS: Slides spotted with S. aureus and 
      treated with methanol and lysozyme were incubated with urea-NaCl reagents on a 
      hot-plate with a precise temperature control and identified with specific DNA 
      probes. CONCLUSIONS: Staphylococcus aureus was detected and differentiated from 
      Staphylococcus epidermidis in 1 h with a novel FISH method that used a single 
      hot-plate and in the absence of dimethyl formamide. SIGNIFICANCE AND IMPACT OF 
      STUDY: A rapid hot-plate FISH assay with urea-NaCl and without toxic dimethyl 
      formamide might be useful if FISH is run infrequently or where resources are 
      limited.
CI  - (c) 2011 The Authors. Letters in Applied Microbiology (c) 2011 The Society for 
      Applied Microbiology.
FAU - Lawson, T S
AU  - Lawson TS
AD  - Faculty of Science, Macquarie University, NSW, Australia. tomxlawson@gmail.com
FAU - Connally, R E
AU  - Connally RE
FAU - Vemulpad, S
AU  - Vemulpad S
FAU - Piper, J A
AU  - Piper JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120106
PL  - England
TA  - Lett Appl Microbiol
JT  - Letters in applied microbiology
JID - 8510094
RN  - 0 (DNA Probes)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 8696NH0Y2X (Dimethylformamide)
RN  - 8W8T17847W (Urea)
RN  - EC 3.2.1.17 (Muramidase)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - DNA Probes
MH  - Dimethylformamide
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Methanol
MH  - Muramidase
MH  - Sodium Chloride/*chemistry
MH  - Species Specificity
MH  - Staphylococcus aureus/genetics/*isolation & purification
MH  - Urea/*chemistry
EDAT- 2011/12/20 06:00
MHDA- 2012/06/23 06:00
CRDT- 2011/12/20 06:00
PHST- 2011/12/20 06:00 [entrez]
PHST- 2011/12/20 06:00 [pubmed]
PHST- 2012/06/23 06:00 [medline]
AID - 10.1111/j.1472-765X.2011.03197.x [doi]
PST - ppublish
SO  - Lett Appl Microbiol. 2012 Mar;54(3):263-6. doi: 10.1111/j.1472-765X.2011.03197.x. 
      Epub 2012 Jan 6.