PMID- 22178696
OWN - NLM
STAT- MEDLINE
DCOM- 20120813
LR  - 20120201
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 73
IP  - 2
DP  - 2012 Feb
TI  - Umbilical cord blood CD34(+)cell-derived progeny produces human leukocyte 
      antigen-G molecules with immuno-modulatory functions.
PG  - 150-5
LID - 10.1016/j.humimm.2011.12.003 [doi]
AB  - Human umbilical cord blood units (UCBs) are an alternative source in 
      allogeneic-stem-cell transplantation. Human leukocyte antigen (HLA)-G is a 
      tolerogenic molecule with a possible implication in UCB immunoregulatory effect. 
      HLA-G expression was observed in UCB myeloid and plasmacytoid dendritic cells; in 
      contrast, CD34(+) cells did not produce this molecule. CD34(+) cells are 
      primitive hematopoietic progenitor cells that are present in UCB and are 
      necessary for long-term engraftment via production of immunoregulatory molecules 
      and a hematopoietic progeny that supports cellular recovery. The role of these 
      cells in UCB transplantation needs further evaluation of HLA-G expression in 
      CD34(+) cells and their hematopoietic progeny. We confirmed the absence of HLA-G 
      expression in CD34(+) cells, whereas CD34(+)-derived progeny secreted HLA-G 
      molecules and expressed HLA-G mRNA in in vitro cultures. Furthermore, soluble HLA 
      (sHLA)-G molecules purified from the culture supernatants of CD34(+)-derived 
      progeny were able to suppress lymphoproliferative response in an HLA-G 
      dose-dependent manner. Overall these results identify CD34(+)-derived 
      hematopoietic progeny as producers of HLA-G molecules and support a role of this 
      antigen as an immuno-modulatory factor in UCB.
CI  - Copyright (c) 2012 American Society for Histocompatibility and Immunogenetics. 
      Published by Elsevier Inc. All rights reserved.
FAU - Buzzi, Marina
AU  - Buzzi M
AD  - Immunohaematology and Transfusion Medicine Service, S.Orsola-Malpighi Hospital, 
      University of Bologna, Bologna, Italy.
FAU - Alviano, Francesco
AU  - Alviano F
FAU - Campioni, Diana
AU  - Campioni D
FAU - Stignani, Marina
AU  - Stignani M
FAU - Melchiorri, Loredana
AU  - Melchiorri L
FAU - Rotola, Antonella
AU  - Rotola A
FAU - Tazzari, Pierluigi
AU  - Tazzari P
FAU - Ricci, Francesca
AU  - Ricci F
FAU - Vaselli, Cristiana
AU  - Vaselli C
FAU - Terzi, Adriana
AU  - Terzi A
FAU - Pagliaro, Pasquale P
AU  - Pagliaro PP
FAU - Cuneo, Antonio
AU  - Cuneo A
FAU - Lanza, Francesco
AU  - Lanza F
FAU - Bontadini, Andrea
AU  - Bontadini A
FAU - Baricordi, Olavio R
AU  - Baricordi OR
FAU - Rizzo, Roberta
AU  - Rizzo R
LA  - eng
PT  - Journal Article
DEP - 20111208
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Antigens, CD34)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Immunologic Factors)
SB  - IM
MH  - Adult
MH  - Antigens, CD34/*immunology
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Fetal Blood/*cytology/*immunology
MH  - Gene Expression Regulation
MH  - HLA-G Antigens/*immunology/isolation & purification/pharmacology
MH  - Humans
MH  - Immunologic Factors/pharmacology
MH  - Pregnancy
EDAT- 2011/12/20 06:00
MHDA- 2012/08/14 06:00
CRDT- 2011/12/20 06:00
PHST- 2011/08/09 00:00 [received]
PHST- 2011/11/15 00:00 [revised]
PHST- 2011/12/01 00:00 [accepted]
PHST- 2011/12/20 06:00 [entrez]
PHST- 2011/12/20 06:00 [pubmed]
PHST- 2012/08/14 06:00 [medline]
AID - S0198-8859(11)00587-8 [pii]
AID - 10.1016/j.humimm.2011.12.003 [doi]
PST - ppublish
SO  - Hum Immunol. 2012 Feb;73(2):150-5. doi: 10.1016/j.humimm.2011.12.003. Epub 2011 
      Dec 8.