PMID- 22185015
OWN - NLM
STAT- MEDLINE
DCOM- 20120127
LR  - 20111221
IS  - 0016-9900 (Print)
IS  - 0016-9900 (Linking)
IP  - 5
DP  - 2011 Sep-Oct
TI  - [Original molecular cytogenetic approach to determining spontaneous chromosomal 
      mutations in the interphase cells to evaluate the mutagenic activity of 
      environmental factors].
PG  - 90-4
AB  - Standard cytogenetic methods are presently used to analyze chromosome anomalies 
      in human somatic cells in order to evaluate the mutagenic activity of 
      environmental factors. Their application is associated with the fact that 
      uncultured cells cannot be analyzed. In this connection there is an urgent need 
      to devise and introduce studies of the number and structure of interphase 
      chromosomes. The paper describes the molecular cytogenetic approach to 
      determining spontaneous chromosomal mutations in the interphase cells to evaluate 
      the mutagenic activity of environmental factors, which is based on the original 
      methods of interphase fluorescence in situ hybridization (FISH) and multicolor 
      chromosome staining and on quantitative FISH and immune FISH. The proposed set of 
      the methods has been tested examining 400000, 200000, and 1600000 embryonic, 
      extraembryonic, and postnatal tissue cells, respectively. The analysis has 
      yielded data on sporadic chromosomal mutations and shown that the proposed 
      complex of the methods can be successfully used to evaluate the mutagenic 
      activity of environmental factors leading to sporadic chromosomal mutations.
FAU - Iurov, I Iu
AU  - Iurov IIu
FAU - Vorsanova, S G
AU  - Vorsanova SG
FAU - Solov'ev, I V
AU  - Solov'ev IV
FAU - Iurov, Iu B
AU  - Iurov IuB
LA  - rus
PT  - English Abstract
PT  - Journal Article
PL  - Russia (Federation)
TA  - Gig Sanit
JT  - Gigiena i sanitariia
JID - 0412700
RN  - 0 (Environmental Pollutants)
RN  - 0 (Mutagens)
SB  - IM
MH  - Brain/embryology/ultrastructure
MH  - Chorion/drug effects/ultrastructure
MH  - Chromosome Aberrations/*chemically induced
MH  - Cytogenetic Analysis/*methods
MH  - Environmental Pollutants/*toxicity
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *Interphase
MH  - Lymphocytes/drug effects/ultrastructure
MH  - Mutagens/*toxicity
EDAT- 2011/12/22 06:00
MHDA- 2012/01/28 06:00
CRDT- 2011/12/22 06:00
PHST- 2011/12/22 06:00 [entrez]
PHST- 2011/12/22 06:00 [pubmed]
PHST- 2012/01/28 06:00 [medline]
PST - ppublish
SO  - Gig Sanit. 2011 Sep-Oct;(5):90-4.