PMID- 22185730
OWN - NLM
STAT- MEDLINE
DCOM- 20120509
LR  - 20111221
IS  - 1945-8932 (Electronic)
IS  - 1945-8932 (Linking)
VI  - 25
IP  - 6
DP  - 2011 Nov-Dec
TI  - Reliability of basophil activation test using CD203c expression in diagnosis of 
      pollen allergy.
PG  - e225-31
LID - 10.2500/ajra.2011.25.3723 [doi]
AB  - BACKGROUND: CD203c is a basophil surface marker and its expression is rapidly 
      up-regulated after cross-linking of high-affinity immunoglobulin E (IgE) receptor 
      (FcepsilonR1) by an allergen. CD203c basophil activation tests have been studied 
      for the in vitro diagnosis of several allergic conditions. However, there is 
      limited data about its diagnostic usefulness. The optimum allergen concentrations 
      for stimulation and allergen specific cutoff values remain unknown for a number 
      of allergens. This study was designed to investigate the efficacy of basophil 
      activation test via CD203c in the diagnosis of pollen allergy. METHODS: The 
      CD203c basophil activation was determined in 31 allergic rhinitis patients with 
      pollen allergy and 9 healthy nonatopic controls during the off-season. CD203c 
      expression was evaluated using three-color staining protocol by flow cytometry. 
      RESULTS: After an in vitro stimulation with grass pollen extract, the CD203c 
      assay clearly discriminated pollen-allergic patients from controls (p < 0.001). A 
      dose-dependent increase in the percentages of CD203c-activated basophils was 
      shown in rhinitis patients with pollen allergy (p < 0.001). The sensitivity and 
      specificity was 100% and optimal cutoff values were 14.05 and 10.05% with 45.1 
      and 4.5 mug/mL Phl p 5 stimulation, respectively. Although the specificity was 
      also 100%, the sensitivity was 93 and 87% and the cutoff values were 5.40 and 
      5.35% with 4.5 x 10(-4) and 4.5 x 10(-5) micrograms/mL Phl p 5 stimulation, 
      respectively. CONCLUSION: The CD203c basophil activation test seems to be a 
      reliable tool in the diagnosis of grass pollen allergy. It could be used when 
      conventional diagnostic tests fail or can not be performed.
FAU - Ozdemir, Secil Kepil
AU  - Ozdemir SK
AD  - Division of Immunology and Allergy Diseases, Ankara University School of 
      Medicine, Ankara, Turkey. secilkepil@gmail.com
FAU - Guloglu, Deniz
AU  - Guloglu D
FAU - Sin, Betul A
AU  - Sin BA
FAU - Elhan, Atilla H
AU  - Elhan AH
FAU - Ikinciogullari, Aydan
AU  - Ikinciogullari A
FAU - Misirligil, Zeynep
AU  - Misirligil Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Rhinol Allergy
JT  - American journal of rhinology & allergy
JID - 101490775
RN  - 0 (ENPP3 protein, human)
RN  - 0 (Phl p V protein, Phleum pratense)
RN  - 0 (Plant Extracts)
RN  - 0 (Plant Proteins)
RN  - EC 3.1.4.- (Phosphoric Diester Hydrolases)
RN  - EC 3.6.1.- (Pyrophosphatases)
SB  - IM
MH  - Adult
MH  - *Basophil Degranulation Test
MH  - Basophils/immunology/*metabolism/pathology
MH  - Cells, Cultured
MH  - Feasibility Studies
MH  - Female
MH  - Humans
MH  - Immunization
MH  - Male
MH  - Middle Aged
MH  - Phosphoric Diester Hydrolases/*analysis
MH  - Plant Extracts/immunology
MH  - Plant Proteins/immunology
MH  - Poaceae
MH  - Pollen/immunology
MH  - Predictive Value of Tests
MH  - Prognosis
MH  - Pyrophosphatases/*analysis
MH  - Rhinitis, Allergic, Seasonal/*diagnosis/immunology/physiopathology
MH  - Sensitivity and Specificity
EDAT- 2011/12/22 06:00
MHDA- 2012/05/10 06:00
CRDT- 2011/12/22 06:00
PHST- 2011/12/22 06:00 [entrez]
PHST- 2011/12/22 06:00 [pubmed]
PHST- 2012/05/10 06:00 [medline]
AID - 10.2500/ajra.2011.25.3723 [doi]
PST - ppublish
SO  - Am J Rhinol Allergy. 2011 Nov-Dec;25(6):e225-31. doi: 10.2500/ajra.2011.25.3723.