PMID- 22186276
OWN - NLM
STAT- MEDLINE
DCOM- 20120906
LR  - 20220316
IS  - 1095-8290 (Electronic)
IS  - 0305-7364 (Print)
IS  - 0305-7364 (Linking)
VI  - 109
IP  - 4
DP  - 2012 Mar
TI  - Amplification, contraction and genomic spread of a satellite DNA family (E180) in 
      Medicago (Fabaceae) and allied genera.
PG  - 773-82
LID - 10.1093/aob/mcr309 [doi]
AB  - BACKGROUND AND AIMS: Satellite DNA is a genomic component present in virtually 
      all eukaryotic organisms. The turnover of highly repetitive satellite DNA is an 
      important element in genome organization and evolution in plants. Here we assess 
      the presence and physical distribution of the repetitive DNA E180 family in 
      Medicago and allied genera. Our goals were to gain insight into the karyotype 
      evolution of Medicago using satellite DNA markers, and to evaluate the taxonomic 
      and phylogenetic signal of a satellite DNA family in a genus hypothesized to have 
      a complex evolutionary history. METHODS: Seventy accessions from Medicago, 
      Trigonella, Melilotus and Trifolium were analysed by PCR to assess the presence 
      of the repetitive E180 family, and fluorescence in situ hybridization (FISH) was 
      used for physical mapping in somatic chromosomes. KEY RESULTS: The E180 repeat 
      unit was PCR-amplified in 37 of 40 taxa in Medicago, eight of 12 species of 
      Trigonella, six of seven species of Melilotus and in two of 11 Trifolium species. 
      Examination of the mitotic chromosomes revealed that only 13 Medicago and two 
      Trigonella species showed FISH signals using the E180 probe. Stronger 
      hybridization signals were observed in subtelomeric and interstitial loci than in 
      the pericentromeric loci, suggesting this satellite family has a preferential 
      genomic location. Not all 13 Medicago species that showed FISH localization of 
      the E180 repeat were phylogenetically related. However, nine of these species 
      belong to the phylogenetically derived clade including the M. sativa and M. 
      arborea complexes. CONCLUSIONS: The use of the E180 family as a phylogenetic 
      marker in Medicago should be viewed with caution. Its amplification appears to 
      have been produced through recurrent and independent evolutionary episodes in 
      both annual and perennial Medicago species as well as in basal and derived 
      clades.
FAU - Rosato, Marcela
AU  - Rosato M
AD  - Jardin Botanico, Universidad de Valencia, Spain.
FAU - Galian, Jose A
AU  - Galian JA
FAU - Rossello, Josep A
AU  - Rossello JA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111219
PL  - England
TA  - Ann Bot
JT  - Annals of botany
JID - 0372347
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Satellite)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - DNA, Plant/*genetics
MH  - DNA, Satellite/*genetics
MH  - *Evolution, Molecular
MH  - Gene Flow
MH  - Genetic Markers
MH  - Medicago/*genetics
MH  - Melilotus/genetics
MH  - Molecular Sequence Data
MH  - Nucleic Acid Amplification Techniques
MH  - Phylogeny
MH  - Repetitive Sequences, Nucleic Acid
MH  - Species Specificity
MH  - Trifolium/genetics
MH  - Trigonella/genetics
PMC - PMC3286279
EDAT- 2011/12/22 06:00
MHDA- 2012/09/07 06:00
PMCR- 2013/03/01
CRDT- 2011/12/22 06:00
PHST- 2011/12/22 06:00 [entrez]
PHST- 2011/12/22 06:00 [pubmed]
PHST- 2012/09/07 06:00 [medline]
PHST- 2013/03/01 00:00 [pmc-release]
AID - mcr309 [pii]
AID - 10.1093/aob/mcr309 [doi]
PST - ppublish
SO  - Ann Bot. 2012 Mar;109(4):773-82. doi: 10.1093/aob/mcr309. Epub 2011 Dec 19.