PMID- 22186418
OWN - NLM
STAT- MEDLINE
DCOM- 20120327
LR  - 20220105
IS  - 1945-7170 (Electronic)
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 153
IP  - 2
DP  - 2012 Feb
TI  - Anxiolytic effects and neuroanatomical targets of estrogen receptor-beta (ERbeta) 
      activation by a selective ERbeta agonist in female mice.
PG  - 837-46
LID - 10.1210/en.2011-1674 [doi]
AB  - The dichotomous anxiogenic and anxiolytic properties of estrogens have been 
      reported to be mediated by two distinct neural estrogen receptors (ER), ERalpha and 
      ERbeta, respectively. Using a combination of pharmacological and genetic approaches, 
      we confirmed that the anxiolytic actions of estradiol are mediated by ERbeta and 
      extended and these observations to demonstrate the neuroanatomical targets 
      involved in ERbeta activation in these behavioral responses. We examined the effects 
      of the biologically active S-enantiomer of diarylpropionitrile (S-DPN) on 
      anxiety-related behavioral measures, the corresponding stress hormonal response 
      to hypothalamo-pituitary-adrenal axis reactivity, and potential sites of neuronal 
      activation in mutant female mice carrying a null mutation for ERbeta gene (betaERKO). 
      S-DPN administration significantly reduced anxiety-like behaviors in the open 
      field, light-dark exploration, and the elevated plus maze (EPM) in ovariectomized 
      wild-type (WT) mice, but not in their betaERKO littermates. Stress-induced 
      corticosterone (CORT) and ACTH were also attenuated by S-DPN in the WT mice but 
      not in the betaERKO mice. Using c-fos induction after elevated plus maze, as a 
      marker of stress-induced neuronal activation, we identified the anterodorsal 
      medial amygdala and bed nucleus of the stria terminalis as the neuronal targets 
      of S-DPN action. Both areas showed elevated c-fos mRNA expression with S-DPN 
      treatment in the WT but not betaERKO females. These studies provide compelling 
      evidence for anxiolytic effects mediated by ERbeta, and its neuroanatomical targets, 
      that send or receive projections to/from the paraventricular nucleus, providing 
      potential indirect mode of action for the control of 
      hypothalamo-pituitary-adrenal axis function and behaviors.
FAU - Oyola, Mario G
AU  - Oyola MG
AD  - Department of Neuroscience, Baylor College of Medicine, One Baylor Plaza, 
      Houston, Texas 77030, USA.
FAU - Portillo, Wendy
AU  - Portillo W
FAU - Reyna, Andrea
AU  - Reyna A
FAU - Foradori, Chad D
AU  - Foradori CD
FAU - Kudwa, Andrea
AU  - Kudwa A
FAU - Hinds, Laura
AU  - Hinds L
FAU - Handa, Robert J
AU  - Handa RJ
FAU - Mani, Shaila K
AU  - Mani SK
LA  - eng
GR  - R01 NS039951/NS/NINDS NIH HHS/United States
GR  - R25 GM069234/GM/NIGMS NIH HHS/United States
GR  - NS039951-11/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20111220
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (2,3-bis(4-hydroxyphenyl)-propionitrile)
RN  - 0 (Anti-Anxiety Agents)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Nitriles)
RN  - 0 (Propionates)
RN  - 0 (beta-Cyclodextrins)
RN  - 1I96OHX6EK (2-Hydroxypropyl-beta-cyclodextrin)
SB  - IM
MH  - 2-Hydroxypropyl-beta-cyclodextrin
MH  - Animals
MH  - Anti-Anxiety Agents/*pharmacology
MH  - Anxiety/metabolism
MH  - Behavior, Animal/*physiology
MH  - Estrogen Receptor beta/*agonists/genetics/metabolism
MH  - Female
MH  - Gene Expression Regulation/physiology
MH  - Mice
MH  - Mice, Knockout
MH  - Mutation
MH  - Nitriles/pharmacology
MH  - Ovariectomy
MH  - Propionates/pharmacology
MH  - Stress, Physiological
MH  - beta-Cyclodextrins/*pharmacology
PMC - PMC3275390
EDAT- 2011/12/22 06:00
MHDA- 2012/03/28 06:00
PMCR- 2013/02/01
CRDT- 2011/12/22 06:00
PHST- 2011/12/22 06:00 [entrez]
PHST- 2011/12/22 06:00 [pubmed]
PHST- 2012/03/28 06:00 [medline]
PHST- 2013/02/01 00:00 [pmc-release]
AID - en.2011-1674 [pii]
AID - EN-11-1674 [pii]
AID - 10.1210/en.2011-1674 [doi]
PST - ppublish
SO  - Endocrinology. 2012 Feb;153(2):837-46. doi: 10.1210/en.2011-1674. Epub 2011 Dec 
      20.