PMID- 22190728
OWN - NLM
STAT- MEDLINE
DCOM- 20120403
LR  - 20211021
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 86
IP  - 5
DP  - 2012 Mar
TI  - Cellular transcription factors induced in trigeminal ganglia during 
      dexamethasone-induced reactivation from latency stimulate bovine herpesvirus 1 
      productive infection and certain viral promoters.
PG  - 2459-73
LID - 10.1128/JVI.06143-11 [doi]
AB  - Bovine herpesvirus 1 (BHV-1), an alphaherpesvirinae subfamily member, establishes 
      latency in sensory neurons. Elevated corticosteroid levels, due to stress, 
      reproducibly triggers reactivation from latency in the field. A single 
      intravenous injection of the synthetic corticosteroid dexamethasone (DEX) to 
      latently infected calves consistently induces reactivation from latency. Lytic 
      cycle viral gene expression is detected in sensory neurons within 6 h after DEX 
      treatment of latently infected calves. These observations suggested that DEX 
      stimulated expression of cellular genes leads to lytic cycle viral gene 
      expression and productive infection. In this study, a commercially available 
      assay-Bovine Gene Chip-was used to compare cellular gene expression in the 
      trigeminal ganglia (TG) of calves latently infected with BHV-1 versus DEX-treated 
      animals. Relative to TG prepared from latently infected calves, 11 cellular genes 
      were induced more than 10-fold 3 h after DEX treatment. Pentraxin three, a 
      regulator of innate immunity and neurodegeneration, was stimulated 35- to 63-fold 
      after 3 or 6 h of DEX treatment. Two transcription factors, promyelocytic 
      leukemia zinc finger (PLZF) and Slug were induced more than 15-fold 3 h after DEX 
      treatment. PLZF or Slug stimulated productive infection 20- or 5-fold, 
      respectively, and Slug stimulated the late glycoprotein C promoter more than 
      10-fold. Additional DEX-induced transcription factors also stimulated productive 
      infection and certain viral promoters. These studies suggest that DEX-inducible 
      cellular transcription factors and/or signaling pathways stimulate lytic cycle 
      viral gene expression, which subsequently leads to successful reactivation from 
      latency in a small subset of latently infected neurons.
FAU - Workman, Aspen
AU  - Workman A
AD  - School of Biological Sciences, University of Nebraska, Lincoln, Lincoln, 
      Nebraska, USA.
FAU - Eudy, James
AU  - Eudy J
FAU - Smith, Lynette
AU  - Smith L
FAU - da Silva, Leticia Frizzo
AU  - da Silva LF
FAU - Sinani, Devis
AU  - Sinani D
FAU - Bricker, Halie
AU  - Bricker H
FAU - Cook, Emily
AU  - Cook E
FAU - Doster, Alan
AU  - Doster A
FAU - Jones, Clinton
AU  - Jones C
LA  - eng
GR  - 1P20RR15635/RR/NCRR NIH HHS/United States
GR  - P20 RR16469/RR/NCRR NIH HHS/United States
GR  - 2P20RR018788-06/RR/NCRR NIH HHS/United States
GR  - T32 AI060547/AI/NIAID NIH HHS/United States
GR  - 1 T32 AIO60547/PHS HHS/United States
GR  - P20 RR016469/RR/NCRR NIH HHS/United States
GR  - P20 RR018788/RR/NCRR NIH HHS/United States
GR  - P20 RR015635/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20111221
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Transcription Factors)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cattle Diseases/*genetics/metabolism/virology
MH  - Cell Line
MH  - Dexamethasone/pharmacology
MH  - Gene Expression Regulation, Viral/*drug effects
MH  - Herpesviridae Infections/genetics/metabolism/*veterinary/virology
MH  - Herpesvirus 1, Bovine/drug effects/genetics/*physiology
MH  - Mice
MH  - Promoter Regions, Genetic/drug effects
MH  - Rabbits
MH  - Transcription Factors/*genetics/metabolism
MH  - Trigeminal Ganglion/*metabolism/virology
MH  - Up-Regulation
MH  - Virus Activation/*drug effects
MH  - *Virus Latency/drug effects
PMC - PMC3302277
EDAT- 2011/12/23 06:00
MHDA- 2012/04/04 06:00
PMCR- 2012/09/01
CRDT- 2011/12/23 06:00
PHST- 2011/12/23 06:00 [entrez]
PHST- 2011/12/23 06:00 [pubmed]
PHST- 2012/04/04 06:00 [medline]
PHST- 2012/09/01 00:00 [pmc-release]
AID - JVI.06143-11 [pii]
AID - 06143-11 [pii]
AID - 10.1128/JVI.06143-11 [doi]
PST - ppublish
SO  - J Virol. 2012 Mar;86(5):2459-73. doi: 10.1128/JVI.06143-11. Epub 2011 Dec 21.