PMID- 22194283
OWN - NLM
STAT- MEDLINE
DCOM- 20120511
LR  - 20111229
IS  - 1460-2377 (Electronic)
IS  - 0953-8178 (Linking)
VI  - 24
IP  - 1
DP  - 2012 Jan
TI  - Unraveling features of the natural MHC class II peptidome of skin-migrated 
      dendritic cells.
PG  - 59-69
LID - 10.1093/intimm/dxr096 [doi]
AB  - Dendritic cells (DCs) migrating from peripheral tissues at steady state are 
      considered the most efficient antigen-presenting cells (APCs) involved in the 
      induction of peripheral T-cell tolerance via self-antigen presentation on MHC 
      class II molecules. However, difficulties in obtaining sufficient numbers of such 
      DCs have precluded previous analyses of their natural MHC class II peptidome in 
      laboratory animals or humans. Here, we overcome this difficulty by collecting the 
      large quantities of sheep DCs that migrate from the skin via the afferent 
      lymphatics at steady state to the draining lymph node. We compared the repertoire 
      of MHC class II-bound peptides from afferent lymph DCs with autologous APCs 
      derived from peripheral blood. A large fraction of the MHC class II peptidome 
      from skin DCs was derived from membrane-recycling proteins (59%) and from 
      proteins of the antigen presentation machinery (50%), whereas these types of 
      peptides constituted a more limited fraction in blood APCs (21 and 11%, 
      respectively). One sheep cytokeratin peptide was identified in the skin DC 
      peptidome indicating active processing of epithelium-derived antigens. 
      Conversely, peptides derived from cytosolic and soluble antigens of the 
      extracellular milieu were more represented in blood APCs than skin DCs. The 
      biased peptidome of skin-migrated DCs indicates that these cells express a 
      peptide repertoire for the generation of self-reactive and/or regulatory T cells 
      mainly directed toward DC molecules from internal and external membranes and to a 
      lesser extent toward antigens of the extracellular milieu, including some 
      tissue-specific peptides.
FAU - Muixi, Laia
AU  - Muixi L
AD  - Immunology Unit, Institut de Biotecnologia i Biomedicina (IBB), Universitat 
      Autonoma de Barcelona (UAB), 08193 Bellaterra, Barcelona, Spain.
FAU - Contreras, Vanessa
AU  - Contreras V
FAU - Collado, Javier A
AU  - Collado JA
FAU - Alexandre, Yannick
AU  - Alexandre Y
FAU - Ballingall, Keith
AU  - Ballingall K
FAU - Bonneau, Michel
AU  - Bonneau M
FAU - Jaraquemada, Dolores
AU  - Jaraquemada D
FAU - Schwartz-Cornil, Isabelle
AU  - Schwartz-Cornil I
LA  - eng
PT  - Journal Article
DEP - 20111222
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (Autoantigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Peptides)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigen-Presenting Cells/immunology/metabolism
MH  - Autoantigens/immunology/metabolism
MH  - Cell Movement/*immunology
MH  - Chromatography, High Pressure Liquid
MH  - Dendritic Cells/*immunology/metabolism
MH  - Female
MH  - Flow Cytometry
MH  - Genotype
MH  - Histocompatibility Antigens Class II/genetics/*immunology/metabolism
MH  - Leukocytes, Mononuclear/immunology/metabolism
MH  - Lymph/immunology/metabolism
MH  - Lymph Nodes/immunology/metabolism
MH  - Mass Spectrometry
MH  - Molecular Sequence Data
MH  - Peptides/*immunology/metabolism
MH  - Proteomics
MH  - Sheep
MH  - Skin/*immunology/metabolism
MH  - T-Lymphocytes/immunology/metabolism
EDAT- 2011/12/24 06:00
MHDA- 2012/05/12 06:00
CRDT- 2011/12/24 06:00
PHST- 2011/12/24 06:00 [entrez]
PHST- 2011/12/24 06:00 [pubmed]
PHST- 2012/05/12 06:00 [medline]
AID - dxr096 [pii]
AID - 10.1093/intimm/dxr096 [doi]
PST - ppublish
SO  - Int Immunol. 2012 Jan;24(1):59-69. doi: 10.1093/intimm/dxr096. Epub 2011 Dec 22.