PMID- 2219730
OWN - NLM
STAT- MEDLINE
DCOM- 19901116
LR  - 20190714
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 179
IP  - 1
DP  - 1990 Nov
TI  - Cloning of the genome of a densovirus and rescue of infectious virions from 
      recombinant plasmid in the insect host Spodoptera littoralis.
PG  - 403-9
AB  - We have cloned an infectious genome of the Junonia coenia densonucleosis virus 
      (JcDNV) into the bacterial plasmid pBR322. The viral genome could be rescued from 
      the recombinant plasmid pBRJ by transfection of pBRJ DNA to sensitive Spodoptera 
      littoralis larvae. pBRJ DNA produced a typical viral infection and a comparable 
      percentage of larvae became infected following inoculation of equivalent amounts 
      of purified virion DNA or cloned viral DNA. Virions extracted from transfected 
      larvae were indistinguishable from wild-type (wt) virions with regard to their 
      biophysical and biological properties. In particular, rescued virions were as 
      infectious as wt virions and showed identical restriction profiles of their 
      genome. In contrast, subcloning of JcDNV DNA deleted at both extremities of a 
      sequence of ca 250 or ca 100 bp resulted in the inability of the recombinant 
      plasmids to initiate a viral infection. These data suggest that, as for 
      vertebrate parvoviruses, the inverted terminal repeats display essential 
      functions in the rescue process and replicative cycle of densoviruses. This is 
      the first report of the molecular cloning of the infectious genome from an insect 
      parvovirus, and more generally from an invertebrate virus. pBRJ should provide an 
      efficient tool to further define the organization of the JcDNV genome and compare 
      it to other parvoviruses.
FAU - Jourdan, M
AU  - Jourdan M
AD  - Station de Recherches de Pathologie Comparee INRA-UA CNRS 1184, Saint 
      Christol-lez-Ales, France.
FAU - Jousset, F X
AU  - Jousset FX
FAU - Gervais, M
AU  - Gervais M
FAU - Skory, S
AU  - Skory S
FAU - Bergoin, M
AU  - Bergoin M
FAU - Dumas, B
AU  - Dumas B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Cloning, Molecular
MH  - DNA Viruses/*genetics
MH  - DNA, Viral/genetics/isolation & purification
MH  - *Genes, Viral
MH  - Insect Viruses/*genetics
MH  - Moths
MH  - Plasmids
MH  - Restriction Mapping
MH  - Transfection
MH  - Viral Proteins/isolation & purification
MH  - Virion/*genetics
EDAT- 1990/11/01 00:00
MHDA- 1990/11/01 00:01
CRDT- 1990/11/01 00:00
PHST- 1990/11/01 00:00 [pubmed]
PHST- 1990/11/01 00:01 [medline]
PHST- 1990/11/01 00:00 [entrez]
AID - 10.1016/0042-6822(90)90308-e [doi]
PST - ppublish
SO  - Virology. 1990 Nov;179(1):403-9. doi: 10.1016/0042-6822(90)90308-e.