PMID- 22197570
OWN - NLM
STAT- MEDLINE
DCOM- 20120508
LR  - 20220317
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 33
IP  - 8
DP  - 2012 Mar
TI  - Mechanical derivation of functional myotubes from adipose-derived stem cells.
PG  - 2482-91
LID - 10.1016/j.biomaterials.2011.12.004 [doi]
AB  - Though reduced serum or myoblast co-culture alone can differentiate 
      adipose-derived stem cells (ASCs) into mesenchymal lineages, efficiency is 
      usually not sufficient to restore function in vivo. Often when injected into 
      fibrotic muscle, their differentiation may be misdirected by the now stiffened 
      tissue. Here ASCs are shown to not just simply reflect the qualitative stiffness 
      sensitivity of bone marrow-derived stem cells (BMSCs) but to exceed BMSC myogenic 
      capacity, expressing the appropriate temporal sequence of muscle transcriptional 
      regulators on muscle-mimicking extracellular matrix in a tension and focal 
      adhesion-dependent manner. ASCs formed multi-nucleated myotubes with a continuous 
      cytoskeleton that was not due to misdirected cell division; microtubule 
      depolymerization severed myotubes, but after washout, ASCs refused at a rate 
      similar to pre-treated values. BMSCs never underwent stiffness-mediated fusion. 
      ASC-derived myotubes, when replated onto non-permissive stiff matrix, maintained 
      their fused state. Together these data imply enhanced mechanosensitivity for 
      ASCs, making them a better therapeutic cell source for fibrotic muscle.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Choi, Yu Suk
AU  - Choi YS
AD  - Department of Bioengineering, University of California, San Diego, CA, USA.
FAU - Vincent, Ludovic G
AU  - Vincent LG
FAU - Lee, Andrew R
AU  - Lee AR
FAU - Dobke, Marek K
AU  - Dobke MK
FAU - Engler, Adam J
AU  - Engler AJ
LA  - eng
GR  - DP2 OD006460/OD/NIH HHS/United States
GR  - DP2 OD006460-01/OD/NIH HHS/United States
GR  - 1DP02 OD006460/OD/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20111223
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Adult
MH  - Biomechanical Phenomena
MH  - Bone Marrow Cells/cytology/metabolism
MH  - Cell Fusion
MH  - Cell Lineage
MH  - Elasticity
MH  - Extracellular Matrix/metabolism
MH  - Focal Adhesions/metabolism
MH  - Gene Expression Regulation
MH  - Giant Cells/cytology/metabolism
MH  - Humans
MH  - Mechanotransduction, Cellular
MH  - Muscle Development
MH  - Muscle Fibers, Skeletal/*cytology/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Stem Cells/*cytology/metabolism
PMC - PMC3261363
MID - NIHMS343520
COIS- Disclosure The authors indicate no potential conflicts of interest.
EDAT- 2011/12/27 06:00
MHDA- 2012/05/09 06:00
PMCR- 2013/03/01
CRDT- 2011/12/27 06:00
PHST- 2011/11/23 00:00 [received]
PHST- 2011/12/02 00:00 [accepted]
PHST- 2011/12/27 06:00 [entrez]
PHST- 2011/12/27 06:00 [pubmed]
PHST- 2012/05/09 06:00 [medline]
PHST- 2013/03/01 00:00 [pmc-release]
AID - S0142-9612(11)01459-1 [pii]
AID - 10.1016/j.biomaterials.2011.12.004 [doi]
PST - ppublish
SO  - Biomaterials. 2012 Mar;33(8):2482-91. doi: 10.1016/j.biomaterials.2011.12.004. 
      Epub 2011 Dec 23.