PMID- 22197815
OWN - NLM
STAT- MEDLINE
DCOM- 20120312
LR  - 20120116
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 417
IP  - 2
DP  - 2012 Jan 13
TI  - Both the Fab and Fc domains of IgG are essential for ROS emission from 
      TNF-alpha-primed neutrophils by IVIG.
PG  - 794-9
LID - 10.1016/j.bbrc.2011.12.038 [doi]
AB  - Intravenous immunoglobulin (IVIG) is currently a very important therapeutic used 
      for not only infectious diseases, but also for autoimmune diseases such as 
      idiopathic thrombocytopenic purpura (ITP). Untoward reactions of IVIG have been 
      thought to result from complement activation by aggregated IgG in IVIG. In 
      addition, the aggregates have been known to activate neutrophils, which may 
      result in the untoward reactions. However, the effect and mechanism of IVIG on 
      neutrophils remain unclear. In this study, we investigated the activation of 
      neutrophils by IVIG in terms of their reactive oxygen species (ROS) emission to 
      elucidate the mechanisms. IVIG-induced ROS emission from purified neutrophils was 
      remarkably augmented by TNF-alpha priming of the cells. The ROS emission from 
      TNF-alpha-primed neutrophils occurred by activation with whole gammaglobulin (GG) 
      molecules, but not F(ab')(2), Fc, or a mixture of F(ab')(2) and Fc. ROS emission 
      by GG was inhibited by the F(ab')(2) fragment and an inhibitory antibody against 
      FcgammaRIII. These results suggest that binding of IVIG to not only surface 
      antigen(s), but also FcgammaRIII on neutrophils, is involved in IVIG-induced ROS 
      emission from TNF-alpha-primed neutrophils, and contribute to the untoward reactions 
      of IVIG.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Higurashi, Saki
AU  - Higurashi S
AD  - Faculty of Pharmaceutical Sciences, Tokyo University of Science, Yamazaki 2641, 
      Noda, Chiba 278-8510, Japan.
FAU - Machino, Yusuke
AU  - Machino Y
FAU - Suzuki, Emiko
AU  - Suzuki E
FAU - Suzuki, Mami
AU  - Suzuki M
FAU - Kohroki, Junya
AU  - Kohroki J
FAU - Masuho, Yasuhiko
AU  - Masuho Y
LA  - eng
PT  - Journal Article
DEP - 20111216
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Immunoglobulin Fab Fragments)
RN  - 0 (Immunoglobulin Fc Fragments)
RN  - 0 (Immunoglobulins, Intravenous)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Cells, Cultured
MH  - Humans
MH  - Immunoglobulin Fab Fragments/*immunology
MH  - Immunoglobulin Fc Fragments/*immunology
MH  - Immunoglobulins, Intravenous/*adverse effects/*immunology
MH  - Neutrophils/*drug effects/immunology
MH  - Reactive Oxygen Species/metabolism
MH  - Respiratory Burst/*drug effects/immunology
MH  - Tumor Necrosis Factor-alpha/immunology
EDAT- 2011/12/27 06:00
MHDA- 2012/03/13 06:00
CRDT- 2011/12/27 06:00
PHST- 2011/12/05 00:00 [received]
PHST- 2011/12/07 00:00 [accepted]
PHST- 2011/12/27 06:00 [entrez]
PHST- 2011/12/27 06:00 [pubmed]
PHST- 2012/03/13 06:00 [medline]
AID - S0006-291X(11)02247-9 [pii]
AID - 10.1016/j.bbrc.2011.12.038 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2012 Jan 13;417(2):794-9. doi: 
      10.1016/j.bbrc.2011.12.038. Epub 2011 Dec 16.