PMID- 22198411
OWN - NLM
STAT- MEDLINE
DCOM- 20120419
LR  - 20220408
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 157
IP  - 3
DP  - 2012 Mar
TI  - Serotype and genotype diversity of infectious bronchitis viruses isolated during 
      1985-2008 in Guangxi, China.
PG  - 467-74
LID - 10.1007/s00705-011-1206-6 [doi]
AB  - The genetic diversity of the hypervariable region I of S1 gene (HVR I) of 
      infectious bronchitis (IB) vaccine strains H120, Ma5 and 4/91 was compared to 
      that of 26 infectious bronchitis virus (IBV) strains isolated from the field in 
      Guangxi province of China during the years 1985-2008, and the field isolates were 
      classified into five major genotypes. Monovalent antisera against three vaccine 
      strains and seven field isolates of different genotypes were prepared by 
      immunizing rabbits with mineral oil adjuvant preparations containing viruses 
      propagated in chicken embryos. Virus neutralization (VN) tests were performed in 
      tracheal organ cultures (TOCs) using these 10 strains with the antisera, and a 
      one-way VN test was then used to compare the relationship of 10 monovalent 
      antisera to the other 19 field isolates. As a result, seven different serotypes 
      were classified based on the results of VN tests with the 26 isolates plus the 
      three vaccine strains. We found that different serotypes were prevalent during 
      different time periods, that more new serotypes have been prevalent in more 
      recent years, and the prevalence of the original dominant serotype has been in 
      constant decline since 2004. In addition, the concordance rate of the 26 field 
      isolates between the S1 genotypes and serotypes was 57.7%.
FAU - Li, Meng
AU  - Li M
AD  - Institute for Poultry Science and Health, Guangxi University, Nanning 530004, 
      Guangxi, China.
FAU - Wang, Xiu-Ying
AU  - Wang XY
FAU - Wei, Ping
AU  - Wei P
FAU - Chen, Qiu-Ying
AU  - Chen QY
FAU - Wei, Zheng-Ji
AU  - Wei ZJ
FAU - Mo, Mei-Lan
AU  - Mo ML
LA  - eng
SI  - GENBANK/DQ859267
SI  - GENBANK/DQ859268
SI  - GENBANK/DQ859269
SI  - GENBANK/DQ859270
SI  - GENBANK/DQ859271
SI  - GENBANK/DQ859272
SI  - GENBANK/DQ859273
SI  - GENBANK/DQ859274
SI  - GENBANK/DQ859275
SI  - GENBANK/EF382348
SI  - GENBANK/EF382349
SI  - GENBANK/EF382350
SI  - GENBANK/EF382351
SI  - GENBANK/EF382352
SI  - GENBANK/EF382353
SI  - GENBANK/EF382354
SI  - GENBANK/EF382355
SI  - GENBANK/EF428324
SI  - GENBANK/EF428325
SI  - GENBANK/EF428326
SI  - GENBANK/EF547363
SI  - GENBANK/EF547364
SI  - GENBANK/FJ770462
SI  - GENBANK/FJ770463
SI  - GENBANK/FJ770465
SI  - GENBANK/FJ770466
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111224
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Antibodies, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral
MH  - Chick Embryo
MH  - Chickens
MH  - China
MH  - Cluster Analysis
MH  - Coronavirus Infections/epidemiology/*veterinary/virology
MH  - *Genetic Variation
MH  - Genotype
MH  - Infectious bronchitis virus/*classification/genetics/immunology/*isolation & 
      purification
MH  - Molecular Sequence Data
MH  - Neutralization Tests
MH  - Phylogeny
MH  - Poultry Diseases/*epidemiology/*virology
MH  - Prevalence
MH  - RNA, Viral/genetics
MH  - Rabbits
MH  - Sequence Analysis, DNA
MH  - Serotyping
EDAT- 2011/12/27 06:00
MHDA- 2012/04/20 06:00
CRDT- 2011/12/27 06:00
PHST- 2011/06/28 00:00 [received]
PHST- 2011/11/23 00:00 [accepted]
PHST- 2011/12/27 06:00 [entrez]
PHST- 2011/12/27 06:00 [pubmed]
PHST- 2012/04/20 06:00 [medline]
AID - 10.1007/s00705-011-1206-6 [doi]
PST - ppublish
SO  - Arch Virol. 2012 Mar;157(3):467-74. doi: 10.1007/s00705-011-1206-6. Epub 2011 Dec 
      24.