PMID- 22199107
OWN - NLM
STAT- MEDLINE
DCOM- 20120703
LR  - 20211021
IS  - 0971-5916 (Print)
IS  - 0971-5916 (Linking)
VI  - 134
IP  - 5
DP  - 2011 Nov
TI  - Prolonged expression of MHC class I - peptide expression in bone marrow derived 
      retrovirus transfected matured dendritic cells by continuous centrifugation in 
      the presence of IL-4.
PG  - 672-8
LID - 10.4103/0971-5916.90993 [doi]
AB  - BACKGROUND & OBJECTIVES: Dendritic cells (DCs) are potent antigen presenting 
      cells which proceed from immature to a mature stage during their differentiation. 
      There are several methods of obtaining long lasting mature antigen expressing DCs 
      and different methods show different levels of antigen expressions. We 
      investigated bone marrow derived DCs for the degree of maturation and genetically 
      engineered antigen presentation in the presence of interleukin-4 (IL-4) as a 
      maturity enhancer. METHODS: DCs and transfected retrovirus were cultured together 
      in the presence of granulocyte-macrophage colony stimulating factor (GMCSF)-IL4, 
      GMCSF +IL4, lipopolysaccharide (LPS). B 7.1, B7.2 and CD11c were measured by the 
      degree of immune fluorescence using enhanced green fluorescent protein (EGFP) 
      shuttled retrovirus transfected antigen. Degree of MHC class I molecule with 
      antigen presentation of antigen was also evaluated by fluorescence activated cell 
      sorting. The antigen presenting capacity of transfected DCs was investigated. 
      Bone marrow DCs were generated in the presence of GMCSF and IL-4 in vitro. 
      Dividing bone marrow cells were infected with EGFP shuttled retrovirus expressing 
      SSP2 by prolonged centrifugation for three consecutive days from day 5, 6 and 7 
      and continued to culture in the presence of GMSCF and IL-4 until day 8. RESULTS: 
      IL-4 as a cytokine increased the maturation of retrovirus transfected DCs by high 
      expression of B 7-1 and B 7-2. Also, IL-4 induced DC enhanced by the prolonged 
      centrifugation and it was shown by increased antigen presentation of these 
      dendric cells as antigen presenting cell (APC). Cytolytic effects were 
      significantly higher in cytotoxic T cell response (CTLs) mixed with transfected 
      DCs than CTLs mixed with pulsed DCs. INTERPRETATION & CONCLUSIONS: There was an 
      enhanced antigen presentation by prolonged expression of antigen loaded MHC class 
      I receptors in DCs in the presence of IL-4 by prolonged centrifugation.
FAU - Hettihewa, L M
AU  - Hettihewa LM
AD  - Department of Pharmacology, Faculty of Medicine, University of Ruhuna, Sri Lanka. 
      menik@med.ruh.ac.lk
LA  - eng
PT  - Journal Article
PL  - India
TA  - Indian J Med Res
JT  - The Indian journal of medical research
JID - 0374701
RN  - 0 (B7 Antigens)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Peptides)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Antigen Presentation/drug effects/immunology
MH  - Antigen-Presenting Cells/immunology/metabolism
MH  - B7 Antigens/genetics/metabolism
MH  - Bone Marrow/*immunology/metabolism
MH  - Cell Differentiation/genetics/immunology
MH  - Centrifugation
MH  - Dendritic Cells/drug effects/*immunology
MH  - Gene Expression Regulation/drug effects/immunology
MH  - Genes, MHC Class I/*immunology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/immunology/*pharmacology
MH  - Humans
MH  - Interleukin-4/immunology/*pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Peptides/genetics/immunology
MH  - Retroviridae/genetics
MH  - T-Lymphocytes, Cytotoxic/drug effects/immunology
MH  - Transfection/methods
PMC - PMC3249966
EDAT- 2011/12/27 06:00
MHDA- 2012/07/04 06:00
PMCR- 2011/11/01
CRDT- 2011/12/27 06:00
PHST- 2011/12/27 06:00 [entrez]
PHST- 2011/12/27 06:00 [pubmed]
PHST- 2012/07/04 06:00 [medline]
PHST- 2011/11/01 00:00 [pmc-release]
AID - IndianJMedRes_2011_134_5_672_90993 [pii]
AID - IJMR-134-672 [pii]
AID - 10.4103/0971-5916.90993 [doi]
PST - ppublish
SO  - Indian J Med Res. 2011 Nov;134(5):672-8. doi: 10.4103/0971-5916.90993.