PMID- 22200086
OWN - NLM
STAT- MEDLINE
DCOM- 20120209
LR  - 20220408
IS  - 2210-7762 (Print)
VI  - 204
IP  - 11
DP  - 2011 Nov
TI  - Microarray-based comparative genomic hybridization of cancer targets reveals 
      novel, recurrent genetic aberrations in the myelodysplastic syndromes.
PG  - 603-28
LID - 10.1016/j.cancergen.2011.10.004 [doi]
AB  - The myelodysplastic syndromes (MDS) are a heterogeneous group of clonal disorders 
      characterized by ineffective hematopoiesis, cytopenias, and a risk of 
      transformation to acute myeloid leukemia (AML). However, only approximately 50% 
      of primary MDS patients show clonal cytogenetic aberrations. To determine whether 
      high-resolution microarray analysis would reveal new or additional aberrations, 
      we analyzed 35 samples derived from patients with a diagnosis or suspicion of MDS 
      and abnormal karyotypes. We used a whole-genome oligonucleotide microarray with 
      targeted coverage of approximately 1900 genes associated with hematologic and 
      other cancers. Clinically relevant copy number aberrations (CNAs) were identified 
      by microarray-based comparative genomic hybridization (aCGH) in all samples 
      (range 1-31, median 5). In 28 of 35 samples (80%), aCGH revealed new cytogenetic 
      aberrations not seen by karyotype or fluorescence in situ hybridization (FISH). 
      Furthermore, 132 cryptic aberrations (</=5 Mb) were identified in 25 cases (71.4%) 
      including deletions of NF1, RUNX1, RASSF1, CCND1, TET2, DNMT3A, HRAS, PDGFRA and 
      FIP1L1. Additionally, aCGH clarified known complex aberrations in 17 of 35 
      samples (48.6%). Finally, our results using whole-genome arrays with higher 
      density coverage targeted to cancer features demonstrate the usefulness of arrays 
      to identify rare and cryptic recurring imbalances that may prove to be 
      significant in disease progression or transformation to AML and may improve the 
      suitability or efficacy of molecularly targeted therapy.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Kolquist, Kathryn A
AU  - Kolquist KA
AD  - Sacred Heart Medical Center, Spokane, WA, USA.
FAU - Schultz, Roger A
AU  - Schultz RA
FAU - Furrow, Aubry
AU  - Furrow A
FAU - Brown, Theresa C
AU  - Brown TC
FAU - Han, Jin-Yeong
AU  - Han JY
FAU - Campbell, Lynda J
AU  - Campbell LJ
FAU - Wall, Meaghan
AU  - Wall M
FAU - Slovak, Marilyn L
AU  - Slovak ML
FAU - Shaffer, Lisa G
AU  - Shaffer LG
FAU - Ballif, Blake C
AU  - Ballif BC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet
JT  - Cancer genetics
JID - 101539150
SB  - IM
MH  - Abnormal Karyotype
MH  - *Chromosome Aberrations
MH  - Comparative Genomic Hybridization/*methods
MH  - Humans
MH  - Myelodysplastic Syndromes/*genetics
MH  - Neoplasms/*genetics
MH  - Oligonucleotide Array Sequence Analysis/*methods
EDAT- 2011/12/28 06:00
MHDA- 2012/02/10 06:00
CRDT- 2011/12/28 06:00
PHST- 2011/09/14 00:00 [received]
PHST- 2011/10/12 00:00 [accepted]
PHST- 2011/12/28 06:00 [entrez]
PHST- 2011/12/28 06:00 [pubmed]
PHST- 2012/02/10 06:00 [medline]
AID - S2210-7762(11)00297-3 [pii]
AID - 10.1016/j.cancergen.2011.10.004 [doi]
PST - ppublish
SO  - Cancer Genet. 2011 Nov;204(11):603-28. doi: 10.1016/j.cancergen.2011.10.004.