PMID- 22200181
OWN - NLM
STAT- MEDLINE
DCOM- 20130221
LR  - 20211021
IS  - 1000-467X (Print)
IS  - 1944-446X (Electronic)
IS  - 1944-446X (Linking)
VI  - 31
IP  - 1
DP  - 2012 Jan
TI  - Tumor necrosis factor-alpha -308G/A polymorphism and risk of hepatocellular 
      carcinoma in hepatitis C virus-infected patients.
PG  - 29-35
LID - 10.5732/cjc.011.10258 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) is an important cytokine in generating an 
      immune response against infection with hepatitis C virus (HCV). The functions of 
      TNF-alpha may be altered by single-nucleotide polymorphisms (SNPs) in its gene 
      structure. We hypothesized that SNPs in TNF-alpha may be important in determining the 
      outcome of an HCV infection. To test this hypothesis, we investigated the role of 
      the polymorphism -308G/A, which is located in the promoter region of the TNF-alpha 
      gene, in the progression of HCV infection in Egyptian patients using a 
      quantitative real-time polymerase chain reaction (qRT-PCR). The distribution of 
      this polymorphism and its impact on the serum level of TNF-alpha was compared between 
      90 HCV-infected patients [45 with HCV-induced cirrhosis and 45 with HCV-related 
      hepatocellular carcinoma (HCC)] and 45 healthy Egyptian volunteers without any 
      history of liver disease. Our results showed that at the TNF-alpha -308 position, the 
      G/G allele was most common (78.5%) in the study population, with the G/A and A/A 
      alleles occurring less frequently (13.3% and 8.1%, respectively). Frequencies of 
      G/G, G/A, and A/A genotypes were 87%, 7%, and 6% in patients with liver cirrhosis 
      and were 94%, 4%, and 2% in patients with HCC, respectively. Serum levels of 
      TNF-alpha were significantly higher in HCV-infected patients than in healthy 
      controls, indicating that the TNF-alpha -308 polymorphism does not influence the 
      production of TNF-alpha. The serum level of TNF-alpha was positively correlated with HCV 
      infection. Taken together, these findings suggest that the TNF-alpha -308 
      polymorphism may not be a host genetic factor associated with the severity of HCV 
      infection, but may be an independent risk factor for HCC.
FAU - Talaat, Roba M
AU  - Talaat RM
AD  - Molecular Biology Department, Menofia University, Sadat, Egypt. 
      Robamtalaat@yahoo.com
FAU - Esmail, Ahmed A
AU  - Esmail AA
FAU - Elwakil, Reda
AU  - Elwakil R
FAU - Gurgis, Adel A
AU  - Gurgis AA
FAU - Nasr, Mahmoud I
AU  - Nasr MI
LA  - eng
PT  - Journal Article
DEP - 20111223
PL  - England
TA  - Chin J Cancer
JT  - Chinese journal of cancer
JID - 101498232
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Alleles
MH  - Carcinoma, Hepatocellular/blood/*genetics/virology
MH  - Female
MH  - Gene Frequency
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - Hepatitis C, Chronic/blood/*genetics
MH  - Humans
MH  - Liver Cirrhosis/blood/genetics
MH  - Liver Neoplasms/blood/*genetics/virology
MH  - Male
MH  - Middle Aged
MH  - *Polymorphism, Single Nucleotide
MH  - Real-Time Polymerase Chain Reaction
MH  - Risk Factors
MH  - Tumor Necrosis Factor-alpha/blood/*genetics
PMC - PMC3777466
EDAT- 2011/12/28 06:00
MHDA- 2013/02/22 06:00
PMCR- 2012/01/01
CRDT- 2011/12/28 06:00
PHST- 2011/12/28 06:00 [entrez]
PHST- 2011/12/28 06:00 [pubmed]
PHST- 2013/02/22 06:00 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - cjc.011.10258 [pii]
AID - cjc-31-01-029 [pii]
AID - 10.5732/cjc.011.10258 [doi]
PST - ppublish
SO  - Chin J Cancer. 2012 Jan;31(1):29-35. doi: 10.5732/cjc.011.10258. Epub 2011 Dec 
      23.