PMID- 22200926
OWN - NLM
STAT- MEDLINE
DCOM- 20120521
LR  - 20160512
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 402
IP  - 5
DP  - 2012 Feb
TI  - TOF-SIMS imaging of halide/thiocyanate anions and hydrogen sulfide in mouse 
      kidney sections using silver-deposited plates.
PG  - 1859-64
LID - 10.1007/s00216-011-5647-2 [doi]
AB  - In vivo imaging of reactive small molecule metabolites with high spatial 
      resolution and specificity could give clues to understanding pathophysiology of 
      various diseases. We herein applied time of flight-secondary ion mass 
      spectrometry (TOF-SIMS) to newly developed silver-deposited plates that were 
      stamped on mouse tissues, and succeeded in visualization of halide (Cl(-), Br(-), 
      and I(-)) and pseudohalide thiocyanate (SCN(-)) anions, a class of substrates for 
      neutrophils/eosinophil peroxidases to produce hypohalous acids (HOX/OX(-) 
      mixture; X: (pseudo)halides), as well as hydrogen sulfide (H(2)S). 
      Forty-micrometer frozen mouse kidney sections on cover glasses were attached to 
      37  degrees C preheated silver-deposited plates and incubated at -10  degrees C for 1 h. After 
      sputter cleaning to remove surface contaminants, the plates were analyzed by 
      TOF-SIMS to identify distribution of Br(-), AgBr(2)(-), I(-), AgI(2)(-), SCN(-), 
      as well as S(2-) and AgS(-) as products of tissue-derived H(2)S. Br(-), 
      AgBr(2)(-), I(-), and SCN(-) anions were mainly distributed in core regions 
      including the inner medulla and inner stripe of the outer medulla (except for 
      I(-)), rather than outer regions such as the cortex and outer stripe of the outer 
      medulla. AgI(2)(-) anion was spread over the whole kidney, although its levels 
      were relatively low. In contrast, S(2-) and AgS(-) anions were mainly present in 
      the outer regions. To our knowledge, this is the first imaging study to reveal 
      the distribution of (pseudo)halides and H(2)S in animal tissue sections.
FAU - Akahoshi, Noriyuki
AU  - Akahoshi N
AD  - Japan Science and Technology Agency (JST), Exploratory Research for Advanced 
      Technology (ERATO), Suematsu Gas Biology Project, Tokyo, Japan.
FAU - Ishizaki, Itsuko
AU  - Ishizaki I
FAU - Naya, Masayuki
AU  - Naya M
FAU - Maekawa, Toshihiko
AU  - Maekawa T
FAU - Yamazoe, Shougo
AU  - Yamazoe S
FAU - Horiuchi, Tadashi
AU  - Horiuchi T
FAU - Kajimura, Mayumi
AU  - Kajimura M
FAU - Ohashi, Yoshiharu
AU  - Ohashi Y
FAU - Suematsu, Makoto
AU  - Suematsu M
FAU - Ishii, Isao
AU  - Ishii I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111227
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Anions)
RN  - 0 (Bromides)
RN  - 0 (Chlorides)
RN  - 0 (Iodides)
RN  - 0 (Thiocyanates)
RN  - 3M4G523W1G (Silver)
RN  - EC 1.11.1.- (Eosinophil Peroxidase)
RN  - YY9FVM7NSN (Hydrogen Sulfide)
SB  - IM
MH  - Animals
MH  - Anions/*analysis
MH  - Bromides/analysis
MH  - Chlorides/analysis
MH  - Eosinophil Peroxidase/metabolism
MH  - Equipment Design
MH  - Hydrogen Sulfide/analysis
MH  - Iodides/analysis
MH  - Kidney/*metabolism/ultrastructure
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Imaging/instrumentation/methods
MH  - Reproducibility of Results
MH  - *Silver
MH  - Spectrometry, Mass, Secondary Ion/*instrumentation/*methods
MH  - Thiocyanates/*analysis
EDAT- 2011/12/28 06:00
MHDA- 2012/05/23 06:00
CRDT- 2011/12/28 06:00
PHST- 2011/10/05 00:00 [received]
PHST- 2011/12/07 00:00 [accepted]
PHST- 2011/11/25 00:00 [revised]
PHST- 2011/12/28 06:00 [entrez]
PHST- 2011/12/28 06:00 [pubmed]
PHST- 2012/05/23 06:00 [medline]
AID - 10.1007/s00216-011-5647-2 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2012 Feb;402(5):1859-64. doi: 10.1007/s00216-011-5647-2. Epub 
      2011 Dec 27.