PMID- 22201473
OWN - NLM
STAT- MEDLINE
DCOM- 20130624
LR  - 20211021
IS  - 1557-7422 (Electronic)
IS  - 1043-0342 (Print)
IS  - 1043-0342 (Linking)
VI  - 23
IP  - 4
DP  - 2012 Apr
TI  - Adeno-associated virus serotype 9 transduction in the central nervous system of 
      nonhuman primates.
PG  - 382-9
LID - 10.1089/hum.2011.200 [doi]
AB  - Widespread distribution of gene products at clinically relevant levels throughout 
      the CNS has been challenging. Adeno-associated virus type 9 (AAV9) vector has 
      been reported as a good candidate for intravascular gene delivery, but low levels 
      of preexisting antibody titers against AAV in the blood abrogate cellular 
      transduction within the CNS. In the present study we compared the effectiveness 
      of vascular delivery and cerebrospinal fluid (CSF) delivery of AAV9 in 
      transducing CNS tissue in nonhuman primates. Both delivery routes generated 
      similar distribution patterns, although we observed a more robust level of 
      transduction after CSF delivery. Consistent with previous reports administering 
      AAV9, we found greater astrocytic than neuronal tropism via both routes, although 
      we did find a greater magnitude of CNS transduction after CSF delivery compared 
      with intravascular delivery. Last, we have demonstrated that delivery of AAV9 
      into the CSF does not shield against AAV antibodies. This has obvious 
      implications when developing and/or implementing any clinical trial studies.
FAU - Samaranch, Lluis
AU  - Samaranch L
AD  - Department of Neurological Surgery, University of California San Francisco, San 
      Francisco, CA 94103, USA.
FAU - Salegio, Ernesto A
AU  - Salegio EA
FAU - San Sebastian, Waldy
AU  - San Sebastian W
FAU - Kells, Adrian P
AU  - Kells AP
FAU - Foust, Kevin D
AU  - Foust KD
FAU - Bringas, John R
AU  - Bringas JR
FAU - Lamarre, Clementine
AU  - Lamarre C
FAU - Forsayeth, John
AU  - Forsayeth J
FAU - Kaspar, Brian K
AU  - Kaspar BK
FAU - Bankiewicz, Krystof S
AU  - Bankiewicz KS
LA  - eng
GR  - 1R01NS073940-01/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120328
PL  - United States
TA  - Hum Gene Ther
JT  - Human gene therapy
JID - 9008950
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
CIN - Hum Gene Ther. 2012 Apr;23(4):340-3. PMID: 22490128
MH  - Animals
MH  - Brain/*metabolism
MH  - Carotid Arteries
MH  - Cisterna Magna
MH  - Dependovirus/*genetics
MH  - Female
MH  - Genetic Therapy
MH  - Genetic Vectors/*administration & dosage
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Infusions, Intravenous
MH  - Macaca fascicularis
MH  - Macaca mulatta
MH  - Male
MH  - Tissue Distribution
MH  - *Transduction, Genetic
PMC - PMC3327605
EDAT- 2011/12/29 06:00
MHDA- 2013/06/26 06:00
PMCR- 2013/04/01
CRDT- 2011/12/29 06:00
PHST- 2011/12/29 06:00 [entrez]
PHST- 2011/12/29 06:00 [pubmed]
PHST- 2013/06/26 06:00 [medline]
PHST- 2013/04/01 00:00 [pmc-release]
AID - 10.1089/hum.2011.200 [pii]
AID - 10.1089/hum.2011.200 [doi]
PST - ppublish
SO  - Hum Gene Ther. 2012 Apr;23(4):382-9. doi: 10.1089/hum.2011.200. Epub 2012 Mar 28.