PMID- 22201908
OWN - NLM
STAT- MEDLINE
DCOM- 20120514
LR  - 20220224
IS  - 1945-0508 (Electronic)
IS  - 1945-0494 (Linking)
VI  - 4
IP  - 2
DP  - 2012 Jan 1
TI  - T-cell phosphokinome as a fingerprint of effective graft versus leukemia.
PG  - 721-33
AB  - Bone marrow and hematopoietic stem cell transplants are given to leukemia 
      patients after chemotherapy and ablative preconditioning, but a significant 
      number will suffer from graft versus host disease (GvHD), where donor immune 
      cells attack recipient tissues. Some graft versus leukemia (GvL) activity 
      protects from leukemia relapse, but determining this balance requires 
      multi-factorial consideration. Genetic and cytokine studies have attempted to 
      improve patient outcome predictions, but there is still far to go. Here, we 
      describe important considerations of the phosphokinome as a fingerprint for 
      predicting GvHD and GvL with partial least squares regression (PLSR) multivariate 
      analysis. Distinguishing factors of GvHD and GvL will first be highlighted to 
      appropriately measure T cell responses to cues that stimulate opposite, 
      orthogonal, and overlapping responses. We will also discuss important kinase 
      signaling cascades predicting cellular responses of cytokine expression, 
      proliferation, and death linked with GvHD or GvL. Higher throughput methods to 
      characterize these signals and different model systems will be discussed, along 
      with benefits and challenges of using the T cell phosphokinome as a fingerprint 
      to predict GvHD and GvL.
FAU - Platt, Manu O
AU  - Platt MO
AD  - Wallace H. Coulter Department of Biomedical Engineering and Institute for 
      Bioengineering and Biosciences, Georgia Institute of Technology and Emory 
      University, Atlanta, GA 30332, USA. manu.platt@bme.gatech.edu
FAU - Kemp, Melissa L
AU  - Kemp ML
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120101
PL  - Singapore
TA  - Front Biosci (Elite Ed)
JT  - Frontiers in bioscience (Elite edition)
JID - 101485240
RN  - 0 (Phosphoproteins)
SB  - IM
MH  - Graft vs Leukemia Effect/*immunology
MH  - Humans
MH  - Least-Squares Analysis
MH  - Phosphoproteins/*immunology
MH  - T-Lymphocytes/*immunology
EDAT- 2011/12/29 06:00
MHDA- 2012/05/15 06:00
CRDT- 2011/12/29 06:00
PHST- 2011/12/29 06:00 [entrez]
PHST- 2011/12/29 06:00 [pubmed]
PHST- 2012/05/15 06:00 [medline]
AID - 413 [pii]
AID - 10.2741/413 [doi]
PST - epublish
SO  - Front Biosci (Elite Ed). 2012 Jan 1;4(2):721-33. doi: 10.2741/413.