PMID- 22205661
OWN - NLM
STAT- MEDLINE
DCOM- 20120613
LR  - 20211021
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 19
IP  - 3
DP  - 2012 Mar
TI  - Effects of pretreating serum samples on the performance of a latex agglutination 
      test for serodiagnosis of paracoccidioidomycosis.
PG  - 386-90
LID - 10.1128/CVI.05274-11 [doi]
AB  - Paracoccidioidomycosis (PCM) is a fungal disease caused by Paracoccidioides 
      brasiliensis, and Brazil is one of the principal countries where it is endemic. 
      Diagnosis is based on the observation of budding P. brasiliensis yeast in 
      clinical specimens from patients; however, the sensitivity of the visualization 
      of fungi is low, indicating that serological tests are used for early diagnosis. 
      The double-immunodiffusion test (ID) is the "gold standard" test for serology in 
      PCM, although the execution of this test requires the availability of 
      laboratorial infrastructure. We report the improved performance of a latex 
      agglutination test (LAT) by pretreating 30 serum samples from PCM patients and 71 
      controls (histoplasmosis and aspergillosis patients, patients with bacterial 
      infections, and normal human sera) with a dilution buffer incubated at 37 degrees C for 
      30 min. The sensitivity and specificity of the LAT test in the nonpretreated 
      samples were 73% and 79%, respectively. However, when samples were pretreated, 
      the sensitivity and specificity of the test increased to 90%. In this study, we 
      did not observe cross-reactivity with histoplasmosis patient sera, but some 
      reactions to sera from patients with aspergillosis and bacterial infections were 
      noted. Normal human sera were not reactive in our tests. These results indicate 
      the need for the elimination of heterologous reactions so that we can adequately 
      use this method for screening cases of PCM.
FAU - Silveira-Gomes, Fabiola
AU  - Silveira-Gomes F
AD  - Science Biologic Institute, Federal University of Para, Belem, Para, Brazil.
FAU - Marques-da-Silva, Silvia Helena
AU  - Marques-da-Silva SH
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111228
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Antibodies, Fungal)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antibodies, Fungal/*blood
MH  - Brazil
MH  - Cross Reactions
MH  - Female
MH  - Humans
MH  - Latex Fixation Tests/methods
MH  - Male
MH  - Middle Aged
MH  - Paracoccidioides/*immunology
MH  - Paracoccidioidomycosis/*diagnosis
MH  - Sensitivity and Specificity
MH  - Serologic Tests/methods
MH  - Serum/*immunology
MH  - Specimen Handling/*methods
PMC - PMC3294624
EDAT- 2011/12/30 06:00
MHDA- 2012/06/14 06:00
PMCR- 2012/09/01
CRDT- 2011/12/30 06:00
PHST- 2011/12/30 06:00 [entrez]
PHST- 2011/12/30 06:00 [pubmed]
PHST- 2012/06/14 06:00 [medline]
PHST- 2012/09/01 00:00 [pmc-release]
AID - CVI.05274-11 [pii]
AID - 05274-11 [pii]
AID - 10.1128/CVI.05274-11 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2012 Mar;19(3):386-90. doi: 10.1128/CVI.05274-11. Epub 2011 
      Dec 28.