PMID- 22205703
OWN - NLM
STAT- MEDLINE
DCOM- 20120409
LR  - 20211021
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 287
IP  - 8
DP  - 2012 Feb 17
TI  - Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 
      (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major 
      allergen from house dust mite.
PG  - 5756-63
LID - 10.1074/jbc.M111.312520 [doi]
AB  - Dendritic cells (DCs) have been shown to play a key role in the initiation and 
      maintenance of immune responses to microbial pathogens as well as to allergens, 
      but the exact mechanisms of their involvement in allergic responses and Th2 cell 
      differentiation have remained elusive. Using retagging, we identified DC-SIGN as 
      a novel receptor involved in the initial recognition and uptake of the major 
      house dust mite and dog allergens Der p 1 and Can f 1, respectively. To confirm 
      this, we used gene silencing to specifically inhibit DC-SIGN expression by DCs 
      followed by allergen uptake studies. Binding and uptake of Der p 1 and Can f 1 
      allergens was assessed by ELISA and flow cytometry. Intriguingly, our data showed 
      that silencing DC-SIGN on DCs promotes a Th2 phenotype in DC/T cell co-cultures. 
      These findings should lead to better understanding of the molecular basis of 
      allergen-induced Th2 cell polarization and in doing so paves the way for the 
      rational design of novel intervention strategies by targeting allergen receptors 
      on innate immune cells or their carbohydrate counterstructures on allergens.
FAU - Emara, Mohamed
AU  - Emara M
AD  - School of Molecular Medical Sciences, Queen's Medical Centre, The University of 
      Nottingham, Nottingham NG7 2UH, United Kingdom.
FAU - Royer, Pierre-Joseph
AU  - Royer PJ
FAU - Mahdavi, Jafar
AU  - Mahdavi J
FAU - Shakib, Farouk
AU  - Shakib F
FAU - Ghaemmaghami, Amir M
AU  - Ghaemmaghami AM
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111228
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Allergens)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Arthropod Proteins)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (ICAM3 protein, human)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (allergen Can f I)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.- (Dermatophagoides pteronyssinus antigen p 1)
SB  - IM
MH  - Allergens/immunology/metabolism
MH  - Animals
MH  - Antigens, CD/*metabolism
MH  - Antigens, Dermatophagoides/immunology/*metabolism
MH  - Arthropod Proteins/immunology/*metabolism
MH  - Biological Transport/drug effects/immunology
MH  - Cell Adhesion Molecules/chemistry/*metabolism/pharmacology
MH  - Coculture Techniques
MH  - Cysteine Endopeptidases/immunology/*metabolism
MH  - Dendritic Cells/cytology/drug effects/immunology/*metabolism
MH  - Dogs
MH  - Dose-Response Relationship, Drug
MH  - Down-Regulation/drug effects
MH  - Humans
MH  - Lectins, C-Type/chemistry/*metabolism
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Protein Binding
MH  - Pyroglyphidae/*immunology
MH  - Receptors, Cell Surface/chemistry/*metabolism
MH  - Solubility
MH  - Staining and Labeling
MH  - Th2 Cells/cytology/drug effects/immunology/metabolism
PMC - PMC3285347
EDAT- 2011/12/30 06:00
MHDA- 2012/04/10 06:00
PMCR- 2011/12/28
CRDT- 2011/12/30 06:00
PHST- 2011/12/30 06:00 [entrez]
PHST- 2011/12/30 06:00 [pubmed]
PHST- 2012/04/10 06:00 [medline]
PHST- 2011/12/28 00:00 [pmc-release]
AID - S0021-9258(20)61232-7 [pii]
AID - M111.312520 [pii]
AID - 10.1074/jbc.M111.312520 [doi]
PST - ppublish
SO  - J Biol Chem. 2012 Feb 17;287(8):5756-63. doi: 10.1074/jbc.M111.312520. Epub 2011 
      Dec 28.