PMID- 22205995
OWN - NLM
STAT- MEDLINE
DCOM- 20120423
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 6
IP  - 12
DP  - 2011
TI  - Detection of murine leukemia virus or mouse DNA in commercial RT-PCR reagents and 
      human DNAs.
PG  - e29050
LID - 10.1371/journal.pone.0029050 [doi]
LID - e29050
AB  - The xenotropic murine leukemia virus (MLV)-related viruses (XMRV) have been 
      reported in persons with prostate cancer, chronic fatigue syndrome, and less 
      frequently in blood donors. Polytropic MLVs have also been described in persons 
      with CFS and blood donors. However, many studies have failed to confirm these 
      findings, raising the possibility of contamination as a source of the positive 
      results. One PCR reagent, Platinum Taq polymerase (pol) has been reported to 
      contain mouse DNA that produces false-positive MLV PCR results. We report here 
      the finding of a large number of PCR reagents that have low levels of MLV 
      sequences. We found that recombinant reverse-transcriptase (RT) enzymes from six 
      companies derived from either MLV or avian myeloblastosis virus contained MLV pol 
      DNA sequences but not gag or mouse DNA sequences. Sequence and phylogenetic 
      analysis showed high relatedness to Moloney MLV, suggesting residual 
      contamination with an RT-containing plasmid. In addition, we identified 
      contamination with mouse DNA and a variety of MLV sequences in commercially 
      available human DNAs from leukocytes, brain tissues, and cell lines. These 
      results identify new sources of MLV contamination and highlight the importance of 
      careful pre-screening of commercial specimens and diagnostic reagents to avoid 
      false-positive MLV PCR results.
FAU - Zheng, HaoQiang
AU  - Zheng H
AD  - Laboratory Branch, Division of HIV/AIDS Prevention, National Center for HIV/AIDS, 
      Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and 
      Prevention, Atlanta, Georgia, United States of America.
FAU - Jia, Hongwei
AU  - Jia H
FAU - Shankar, Anupama
AU  - Shankar A
FAU - Heneine, Walid
AU  - Heneine W
FAU - Switzer, William M
AU  - Switzer WM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20111220
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA, Viral)
RN  - 0 (Indicators and Reagents)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - *DNA Contamination
MH  - DNA, Viral/*analysis
MH  - False Positive Reactions
MH  - Humans
MH  - Indicators and Reagents
MH  - Leukemia Virus, Murine/enzymology/*genetics
MH  - Mice
MH  - Plasmids/genetics
MH  - *RNA-Directed DNA Polymerase
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
PMC - PMC3243700
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2011/12/30 06:00
MHDA- 2012/04/24 06:00
PMCR- 2011/12/20
CRDT- 2011/12/30 06:00
PHST- 2011/10/03 00:00 [received]
PHST- 2011/11/18 00:00 [accepted]
PHST- 2011/12/30 06:00 [entrez]
PHST- 2011/12/30 06:00 [pubmed]
PHST- 2012/04/24 06:00 [medline]
PHST- 2011/12/20 00:00 [pmc-release]
AID - PONE-D-11-19419 [pii]
AID - 10.1371/journal.pone.0029050 [doi]
PST - ppublish
SO  - PLoS One. 2011;6(12):e29050. doi: 10.1371/journal.pone.0029050. Epub 2011 Dec 20.