PMID- 22210270
OWN - NLM
STAT- MEDLINE
DCOM- 20130507
LR  - 20211021
IS  - 1437-160X (Electronic)
IS  - 0172-8172 (Linking)
VI  - 32
IP  - 12
DP  - 2012 Dec
TI  - OPG inhibits gene expression of RANK and CAII in mouse osteoclast-like cell.
PG  - 3993-8
LID - 10.1007/s00296-011-2338-4 [doi]
AB  - This study was designed to determine the effects of the osteoprotegerin (OPG) on 
      the mRNA expression of carbonic anhydrase II (CAII) and the receptor activator of 
      NF-kappaB (RANK) in mouse osteoclast-like cells. Marrow cells were harvested from 
      femora and tibiae of mouse and cultured in 6-well chamber slides. After 1 day of 
      incubation, the marrow cells were exposed to M-CSF (25 ng/ml), RANKL (50 ng/ml), 
      and different concentrations of OPG (50, 75, and 100 ng/ml, respectively) for 3 
      days. Osteoclast-like cells were confirmed by both tartrate-resistant acid 
      phosphatase (TRAP) stain and bone resorption assay. The expression of RANK and 
      CAIImRNA was determined with real-time fluorescent quantitative polymerase chain 
      reaction. The numbers of multinucleated, TRAP-positive osteoclast-like cells, and 
      resorption pits formed were observed. Compared with the M-CSF + RANKL group, 
      RANKmRNA expression was statistically decreased in the M-CSF and M-CSF + RANKL + 
      OPG (100 ng/ml) groups (P = 0.004, P = 0.024, respectively); Compared with the 
      M-CSF, M-CSF + RANKL, and M-CSF + RANKL + OPG (100 ng/ml) group, CAIImRNA 
      expression in the M-CSF + RANKL + OPG (75 ng/ml) groups was statistically 
      decreased (P = 0.001, P = 0.008, and P = 0.036, respectively). These data suggest 
      that OPG could regulate the expression of RANK and CA II mRNA in the marrow 
      culture system.
FAU - Chen, Jian
AU  - Chen J
AD  - Department of Rehabilitation, Zhong Shang Hospital, Xiamen University, Xiamen 
      361004, China. chenjian5@medmail.com.cn
FAU - He, Jian-Quan
AU  - He JQ
FAU - Zhen, Shu-Yu
AU  - Zhen SY
FAU - Huang, Li-Qun
AU  - Huang LQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111231
PL  - Germany
TA  - Rheumatol Int
JT  - Rheumatology international
JID - 8206885
RN  - 0 (Osteoprotegerin)
RN  - 0 (Receptor Activator of Nuclear Factor-kappa B)
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
SB  - IM
MH  - Animals
MH  - Bone and Bones/cytology/drug effects/metabolism
MH  - Carbonic Anhydrase II/*genetics/metabolism
MH  - Cells, Cultured
MH  - Gene Expression/*drug effects
MH  - Mice
MH  - Osteoclasts/cytology/*drug effects/metabolism
MH  - Osteoprotegerin/*pharmacology
MH  - Receptor Activator of Nuclear Factor-kappa B/*genetics/metabolism
EDAT- 2012/01/03 06:00
MHDA- 2013/05/08 06:00
CRDT- 2012/01/03 06:00
PHST- 2011/08/08 00:00 [received]
PHST- 2011/12/20 00:00 [accepted]
PHST- 2012/01/03 06:00 [entrez]
PHST- 2012/01/03 06:00 [pubmed]
PHST- 2013/05/08 06:00 [medline]
AID - 10.1007/s00296-011-2338-4 [doi]
PST - ppublish
SO  - Rheumatol Int. 2012 Dec;32(12):3993-8. doi: 10.1007/s00296-011-2338-4. Epub 2011 
      Dec 31.