PMID- 22211589
OWN - NLM
STAT- MEDLINE
DCOM- 20121016
LR  - 20120618
IS  - 1574-695X (Electronic)
IS  - 0928-8244 (Linking)
VI  - 65
IP  - 2
DP  - 2012 Jul
TI  - The microorganisms in chronically infected end-stage and non-end-stage cystic 
      fibrosis patients.
PG  - 236-44
LID - 10.1111/j.1574-695X.2011.00925.x [doi]
AB  - Patients suffering from cystic fibrosis (CF) develop chronic lung infections 
      because of highly viscous mucus, where bacteria can form biofilms. In this study, 
      we investigated the microorganisms present in the lungs of end-stage and 
      non-end-stage patients using standard culturing techniques and molecular methods. 
      Tissue and sputum samples (n = 34) from explanted lungs of five end-stage 
      patients were examined along with routine expectorates (n = 15) from 13 patients 
      with non-end-stage CF, representing earlier stages of chronic lung infections. 
      Previously, using peptide nucleic acid (PNA) fluorescence in situ hybridization 
      (FISH), we have shown that Pseudomonas aeruginosa was the sole pathogen in 
      end-stage CF lungs (Pediatr Pulmonol 2009, 44: 547). In this study, this tendency 
      was supported by the results of real-time PCR, confirming previous results 
      obtained by standard culturing and 16S rRNA gene analysis (J Clin Microbiol 2011, 
      49: 4352). Conversely, the non-end-stage patients were found to harbor several 
      species by culturing. PNA FISH confirmed heterogeneous microbiota and showed that 
      the bacteria were located in monospecies aggregates with no apparent physical 
      interaction between the different microcolonies. In conclusion, standard 
      culturing identifies the dominating pathogens, which seem to reside in 
      monospecies microcolonies. The possibility of signaling between the distinct 
      microcolonies still has to be verified and elucidated.
CI  - (c) 2011 Federation of European Microbiological Societies. Published by Blackwell 
      Publishing Ltd. All rights reserved.
FAU - Rudkjobing, Vibeke B
AU  - Rudkjobing VB
AD  - Department of Biotechnology, Chemistry, and Environmental Engineering, Faculty of 
      Engineering and Science, Aalborg University, Aalborg, Denmark.
FAU - Thomsen, Trine R
AU  - Thomsen TR
FAU - Alhede, Morten
AU  - Alhede M
FAU - Kragh, Kasper N
AU  - Kragh KN
FAU - Nielsen, Per H
AU  - Nielsen PH
FAU - Johansen, Ulla R
AU  - Johansen UR
FAU - Givskov, Michael
AU  - Givskov M
FAU - Hoiby, Niels
AU  - Hoiby N
FAU - Bjarnsholt, Thomas
AU  - Bjarnsholt T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120123
PL  - England
TA  - FEMS Immunol Med Microbiol
JT  - FEMS immunology and medical microbiology
JID - 9315554
SB  - IM
MH  - Bacteria/*classification/*isolation & purification
MH  - Bacteriological Techniques
MH  - *Biodiversity
MH  - Cystic Fibrosis/*complications
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung/microbiology
MH  - Pneumonia, Bacterial/*microbiology
MH  - Real-Time Polymerase Chain Reaction
MH  - Sputum/microbiology
EDAT- 2012/01/04 06:00
MHDA- 2012/10/17 06:00
CRDT- 2012/01/04 06:00
PHST- 2011/11/02 00:00 [received]
PHST- 2011/12/12 00:00 [revised]
PHST- 2011/12/13 00:00 [accepted]
PHST- 2012/01/04 06:00 [entrez]
PHST- 2012/01/04 06:00 [pubmed]
PHST- 2012/10/17 06:00 [medline]
AID - 10.1111/j.1574-695X.2011.00925.x [doi]
PST - ppublish
SO  - FEMS Immunol Med Microbiol. 2012 Jul;65(2):236-44. doi: 
      10.1111/j.1574-695X.2011.00925.x. Epub 2012 Jan 23.