PMID- 22211939
OWN - NLM
STAT- MEDLINE
DCOM- 20120615
LR  - 20220309
IS  - 1574-6968 (Electronic)
IS  - 0378-1097 (Linking)
VI  - 328
IP  - 2
DP  - 2012 Mar
TI  - Identification of Chromera velia by fluorescence in situ hybridization.
PG  - 144-9
LID - 10.1111/j.1574-6968.2011.02489.x [doi]
AB  - Chromera velia is evolutionarily the closest free-living and photosynthetic 
      organism to the medically important obligatory parasitic apicomplexans that cause 
      diseases including malaria and toxoplasmosis. In this study, a novel 
      oligonucleotide probe targeting C. velia's small subunit ribosomal RNA was 
      designed. To enable usage of this probe as a detection tool, a fluorescence in 
      situ hybridization (FISH) protocol was optimized. The results obtained showed 
      that when used in combination, the C. velia CV1 probe and optimized FISH protocol 
      enabled efficient detection of C. velia in culture. This new technique will allow 
      a better understanding of the ecological role of C. velia within the coral 
      microhabitat.
CI  - (c) 2011 Federation of European Microbiological Societies. Published by Blackwell 
      Publishing Ltd. All rights reserved.
FAU - Morin-Adeline, Victoria
AU  - Morin-Adeline V
AD  - Faculty of Veterinary Science, University of Sydney, Sydney, NSW, Australia.
FAU - Foster, Christie
AU  - Foster C
FAU - Slapeta, Jan
AU  - Slapeta J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120119
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (Culture Media)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Anthozoa/*parasitology
MH  - Apicomplexa/chemistry/genetics/growth & development/*isolation & purification
MH  - Base Sequence
MH  - Culture Media/chemistry
MH  - DNA/chemistry/isolation & purification
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Oligonucleotide Probes/*chemistry
MH  - RNA, Ribosomal/genetics
MH  - Sequence Alignment
EDAT- 2012/01/04 06:00
MHDA- 2012/06/16 06:00
CRDT- 2012/01/04 06:00
PHST- 2011/11/02 00:00 [received]
PHST- 2011/12/19 00:00 [revised]
PHST- 2011/12/19 00:00 [accepted]
PHST- 2012/01/04 06:00 [entrez]
PHST- 2012/01/04 06:00 [pubmed]
PHST- 2012/06/16 06:00 [medline]
AID - 10.1111/j.1574-6968.2011.02489.x [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 2012 Mar;328(2):144-9. doi: 
      10.1111/j.1574-6968.2011.02489.x. Epub 2012 Jan 19.