PMID- 22213113
OWN - NLM
STAT- MEDLINE
DCOM- 20120717
LR  - 20230207
IS  - 1549-4918 (Electronic)
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 30
IP  - 4
DP  - 2012 Apr
TI  - Thermal stability of fibroblast growth factor protein is a determinant factor in 
      regulating self-renewal, differentiation, and reprogramming in human pluripotent 
      stem cells.
PG  - 623-30
LID - 10.1002/stem.1021 [doi]
AB  - Fibroblast growth factor (FGF), transforming growth factor (TGF)/Nodal, and 
      Insulin/insulin-like growth factor (IGF) signaling pathways are sufficient to 
      maintain human embryonic stem cells (ESCs) and induced pluripotent stem cells in 
      a proliferative, undifferentiated state. Here, we show that only a few FGF family 
      members (FGF2, FGF4, FGF6, and FGF9) are able to sustain strong 
      extracellular-signal-regulated kinase (ERK) phosphorylation and NANOG expression 
      levels in human ESCs. Surprisingly, FGF1, which is reported to target the same 
      set of receptors as FGF2, fails to sustain ERK phosphorylation and NANOG 
      expression under standard culture conditions. We find that the failure of FGF1 to 
      sustain ES is due to thermal instability of the wild-type protein, not receptor 
      specificity, and that a mutated thermal-stable FGF1 sustains human ESCs and 
      supports both differentiation and reprogramming protocols.
CI  - Copyright (c) 2011 AlphaMed Press.
FAU - Chen, Guokai
AU  - Chen G
AD  - Morgridge Institute for Research, Madison, Wisconsin, USA.
FAU - Gulbranson, Daniel R
AU  - Gulbranson DR
FAU - Yu, Pengzhi
AU  - Yu P
FAU - Hou, Zhonggang
AU  - Hou Z
FAU - Thomson, James A
AU  - Thomson JA
LA  - eng
GR  - U01 ES017166/ES/NIEHS NIH HHS/United States
GR  - U01ES017166/ES/NIEHS NIH HHS/United States
GR  - RR-05-19/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Mutant Proteins)
RN  - 0 (Receptors, Fibroblast Growth Factor)
RN  - 62031-54-3 (Fibroblast Growth Factors)
RN  - 9005-49-6 (Heparin)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Cell Differentiation/*drug effects
MH  - Cell Proliferation/drug effects
MH  - Cellular Reprogramming/*drug effects
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Fibroblast Growth Factors/metabolism/*pharmacology
MH  - Heparin/metabolism
MH  - Humans
MH  - Mutant Proteins/metabolism
MH  - Mutation/genetics
MH  - Phosphorylation/drug effects
MH  - Pluripotent Stem Cells/*cytology/*drug effects/enzymology
MH  - Protein Stability/drug effects
MH  - Receptors, Fibroblast Growth Factor/metabolism
MH  - *Temperature
PMC - PMC3538808
MID - NIHMS428275
COIS- Disclosure of Potential Conflicts of Interest J.A.T. is a founder, stockowner, 
      consultant, and board member of Cellular Dynamics International (CDI). He also 
      serves as scientific advisor to and has financial interests in Tactics II Stem 
      Cell Ventures.
EDAT- 2012/01/04 06:00
MHDA- 2012/07/18 06:00
PMCR- 2013/01/08
CRDT- 2012/01/04 06:00
PHST- 2012/01/04 06:00 [entrez]
PHST- 2012/01/04 06:00 [pubmed]
PHST- 2012/07/18 06:00 [medline]
PHST- 2013/01/08 00:00 [pmc-release]
AID - 10.1002/stem.1021 [doi]
PST - ppublish
SO  - Stem Cells. 2012 Apr;30(4):623-30. doi: 10.1002/stem.1021.