PMID- 22213140
OWN - NLM
STAT- MEDLINE
DCOM- 20120917
LR  - 20201209
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 113
IP  - 5
DP  - 2012 May
TI  - Odontogenic ameloblasts-associated protein (ODAM), via phosphorylation by bone 
      morphogenetic protein receptor type IB (BMPR-IB), is implicated in ameloblast 
      differentiation.
PG  - 1754-65
LID - 10.1002/jcb.24047 [doi]
AB  - To elucidate the function of the odontogenic ameloblast-associated protein (ODAM) 
      in ameloblasts, we identified more than 74 proteins that interact with ODAM using 
      protoarray. Of the identified proteins, bone morphogenetic protein receptor 
      type-IB (BMPR-IB) was physiologically relevant in differentiating ameloblasts. 
      ODAM and BMPR-IB exhibited similar patterns of expression in vitro, during 
      ameloblast differentiation. ODAM and BMPR-IB interacted through the C-terminus of 
      ODAM, which resulted in increased ODAM phosphorylation in the presence of bone 
      morphogenetic protein 2 (BMP-2). Immunoprecipitation assays using Ser-Xaa-Glu 
      (SXE) mutants of ODAM demonstrated that the phosphorylation of ODAM by BMPR-IB 
      occurs at this motif, and this phosphorylation is required for the activation of 
      MAPKs. ODAM phosphorylation was detected in ameloblasts during ameloblast 
      differentiation and enamel mineralization in vitro and involved in the activation 
      of downstream factors of MAPKs. Therefore, the BMP-2-BMPR-IB-ODAM-MAPK signaling 
      cascade has important roles in ameloblast differentiation and enamel 
      mineralization. Our data suggest that ODAM facilitates the progression of tooth 
      development in cooperation with BMPR-IB through distinct domains of ODAM.
CI  - Copyright (c) 2011 Wiley Periodicals, Inc.
FAU - Lee, Hye-Kyung
AU  - Lee HK
AD  - Department of Oral Histology-Developmental Biology, School of Dentistry and 
      Dental Research Institute, BK 21, Seoul National University, Seoul 110-749, 
      Korea.
FAU - Park, Jong-Tae
AU  - Park JT
FAU - Cho, Young-Sik
AU  - Cho YS
FAU - Bae, Hyun-Sook
AU  - Bae HS
FAU - Cho, Moon-Il
AU  - Cho MI
FAU - Park, Joo-Cheol
AU  - Park JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Bmp2 protein, mouse)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Mutant Proteins)
RN  - 0 (ODAM protein, mouse)
RN  - 0 (Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.7.11.30 (Bmpr1b protein, mouse)
RN  - EC 2.7.11.30 (Bone Morphogenetic Protein Receptors, Type I)
SB  - IM
MH  - Ameloblasts/*cytology/drug effects/*metabolism
MH  - Animals
MH  - Bone Morphogenetic Protein 2/pharmacology
MH  - Bone Morphogenetic Protein Receptors, Type I/antagonists & 
      inhibitors/chemistry/genetics/*metabolism
MH  - Cell Differentiation/physiology
MH  - Cell Line
MH  - HEK293 Cells
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - MAP Kinase Signaling System
MH  - Mice
MH  - Mutagenesis
MH  - Mutant Proteins/chemistry/genetics/metabolism
MH  - Odontogenesis/genetics/physiology
MH  - Phosphorylation
MH  - Protein Interaction Domains and Motifs
MH  - Proteins/chemistry/genetics/*metabolism
MH  - RNA, Small Interfering/genetics
MH  - Signal Transduction
MH  - Transfection
EDAT- 2012/01/04 06:00
MHDA- 2012/09/18 06:00
CRDT- 2012/01/04 06:00
PHST- 2012/01/04 06:00 [entrez]
PHST- 2012/01/04 06:00 [pubmed]
PHST- 2012/09/18 06:00 [medline]
AID - 10.1002/jcb.24047 [doi]
PST - ppublish
SO  - J Cell Biochem. 2012 May;113(5):1754-65. doi: 10.1002/jcb.24047.