PMID- 22215471
OWN - NLM
STAT- MEDLINE
DCOM- 20120731
LR  - 20211021
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Print)
IS  - 1058-0468 (Linking)
VI  - 29
IP  - 2
DP  - 2012 Feb
TI  - Sperm chromosomal aneuploidy and DNA integrity of infertile men with 
      anejaculation.
PG  - 185-94
LID - 10.1007/s10815-011-9688-4 [doi]
AB  - PURPOSE: To explore sperm chromosomal aneuploidy, sperm membrane and DNA 
      integrity in infertile patients with anejaculation. METHODS: Semen samples were 
      collected from 18 infertile men with spinal cord injury (SCI) by penile vibratory 
      stimulation (PVS) and from 14 psychogenic anejaculation (PA) patients by 
      percutaneous vasal sperm aspiration (PVSA). These semen samples as well as 
      samples from 16 donors were analyzed using the hypo-osmotic swelling (HOS) test, 
      the sperm chromatin dispersion (SCD) test and multi-color fluorescence in situ 
      hybridization (FISH) with probes specific for chromosomes 13, 18, 21, X and Y. 
      RESULTS: There were significant differences in the percentages of motile sperm, 
      normal morphologic sperm and sperm DNA fragmentation between the infertile men 
      with SCI and the control group (P < 0.05 and P < 0.01). The sperm motility was 
      significantly greater in the PA-PVSA group than in the SCI-PVS group (P < 0.01). 
      The number of round cells per mL of semen obtained from the 18 SCI patients by 
      PVS was between 1 and 8 million. The rate of sperm DNA fragmentation in the 
      SCI-PVS group was higher than that of the PA-PVSA group (P < 0.05). The 
      aneuploidy rates for the SCI patients were 2.4-fold higher for chromosomes 13, 18 
      and 21 and 2.2-fold higher for chromosomes X and Y than for patients in the 
      control group (P < 0.0001). CONCLUSIONS: The semen quality is poorer, sperm DNA 
      fragmentation and sperm chromosomal aneuploidies are seen at a higher rate for 
      SCI patients compared to healthy, fertile and normospermic men. Whether the 
      difference in yield is due to increased scrotal temperature, genitourinary 
      infection, or other reasons requires further study.
FAU - Qiu, Yi
AU  - Qiu Y
AD  - Key Laboratory for Improving Birth Outcome Technique, Shandong Provincial Family 
      Planning Institute of Science and Technology, Jinan, Shandong, China. 
      qiuyi987@sina.com
FAU - Wang, Lei-Guang
AU  - Wang LG
FAU - Zhang, Li-Hong
AU  - Zhang LH
FAU - Li, Juan
AU  - Li J
FAU - Zhang, Ai-Dong
AU  - Zhang AD
FAU - Zhang, Mei-Hua
AU  - Zhang MH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120104
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
SB  - IM
MH  - *Aneuploidy
MH  - Chromosomes, Human/genetics
MH  - DNA Fragmentation
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infertility, Male/etiology/*genetics
MH  - Male
MH  - *Sperm Motility
MH  - Spermatozoa/*cytology
MH  - Spinal Cord Injuries/complications
PMC - PMC3270129
EDAT- 2012/01/05 06:00
MHDA- 2012/08/01 06:00
PMCR- 2013/02/01
CRDT- 2012/01/05 06:00
PHST- 2011/06/25 00:00 [received]
PHST- 2011/11/30 00:00 [accepted]
PHST- 2012/01/05 06:00 [entrez]
PHST- 2012/01/05 06:00 [pubmed]
PHST- 2012/08/01 06:00 [medline]
PHST- 2013/02/01 00:00 [pmc-release]
AID - 9688 [pii]
AID - 10.1007/s10815-011-9688-4 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2012 Feb;29(2):185-94. doi: 10.1007/s10815-011-9688-4. 
      Epub 2012 Jan 4.