PMID- 22215941
OWN - NLM
STAT- MEDLINE
DCOM- 20120302
LR  - 20211021
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Print)
IS  - 1007-9327 (Linking)
VI  - 17
IP  - 47
DP  - 2011 Dec 21
TI  - Five methods for detection of Helicobacter pylori in the Turkish population.
PG  - 5172-6
LID - 10.3748/wjg.v17.i47.5172 [doi]
AB  - AIM: To compare culture analysis, Helicobacter pylori (H. pylori) stool antigen 
      (HpSA) test, polymerase chain reaction (PCR) and fluorescence in situ 
      hybridization (FISH) for H. pylori detection. METHODS: One hundred and thirty-two 
      consecutive adult dyspeptic patients receiving diagnostic endoscopy at the 
      department of gastroenterology were enrolled in this study. Culture and 
      histological examination were performed on biopsy specimens. PCR and FISH tests 
      were applied to histopathological samples. Stool samples that were simultaneously 
      collected were tested for the H. pylori antigen using the HpSA test and bacterial 
      DNA using stool PCR. RESULTS: H. pylori was positively identified by histological 
      examination in 85/132 (64.4%) of the patients, while positive samples were found 
      in 56 (42.4%), 64 (48.5%), 98 (74.2%), 28 (21.2%) and 81 (61.4%) of the patients 
      by culture, HpSA, PCR, stool PCR and FISH methods, respectively. The results of 
      the culture, biopsy PCR, HpSA and FISH tests, with the exception of the stool 
      PCR, were found to correlate with the histological examination as a gold 
      standard. CONCLUSION: The HpSA test is a rapid, simple, and noninvasive test for 
      monitoring therapy. FISH is an accurate, rapid, cost-effective, and easy-to-use 
      test for H. pylori detection.
FAU - Aktepe, Orhan Cem
AU  - Aktepe OC
AD  - Department of Microbiology, Faculty of Medicine, Afyon Kocatepe University, Ali 
      Cetinkaya Campus, 03200 Afyonkarahisar, Turkey. aktepef@hotmail.com
FAU - Ciftci, Ihsan Hakki
AU  - Ciftci IH
FAU - Safak, Birol
AU  - Safak B
FAU - Uslan, Ihsan
AU  - Uslan I
FAU - Dilek, Fatma Husniye
AU  - Dilek FH
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Antigens, Bacterial)
SB  - IM
MH  - Adult
MH  - Antigens, Bacterial/*analysis
MH  - Diagnostic Tests, Routine/economics/*methods/standards
MH  - Feces/microbiology
MH  - Helicobacter Infections/*diagnosis/microbiology
MH  - Helicobacter pylori/genetics/*immunology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Polymerase Chain Reaction/methods
MH  - Turkey
PMC - PMC3243883
OTO - NOTNLM
OT  - Fluorescence in situ hybridization
OT  - Helicobacter pylori
OT  - Helicobacter pylori stool antigen
OT  - Histology
OT  - Polymerase chain reaction
EDAT- 2012/01/05 06:00
MHDA- 2012/03/03 06:00
PMCR- 2011/12/21
CRDT- 2012/01/05 06:00
PHST- 2011/01/23 00:00 [received]
PHST- 2011/04/19 00:00 [revised]
PHST- 2011/04/26 00:00 [accepted]
PHST- 2012/01/05 06:00 [entrez]
PHST- 2012/01/05 06:00 [pubmed]
PHST- 2012/03/03 06:00 [medline]
PHST- 2011/12/21 00:00 [pmc-release]
AID - 10.3748/wjg.v17.i47.5172 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2011 Dec 21;17(47):5172-6. doi: 10.3748/wjg.v17.i47.5172.