PMID- 22219324
OWN - NLM
STAT- MEDLINE
DCOM- 20120611
LR  - 20120120
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 188
IP  - 3
DP  - 2012 Feb 1
TI  - Vitamin D receptor gene polymorphisms and HLA DRB1*04 cosegregation in Saudi type 
      2 diabetes patients.
PG  - 1325-32
LID - 10.4049/jimmunol.1101954 [doi]
AB  - The vitamin D receptor (VDR) gene has been involved in the modulation of 
      susceptibility to inflammatory and autoimmune conditions, and could play a role 
      in the pathogenesis of type 2 diabetes mellitus (T2DM). Susceptibility to T2DM 
      was recently also suggested to associate with HLA alleles. We evaluated possible 
      correlations between VDR polymorphisms, HLA alleles, and risk for development of 
      T2DM by analyzing 627 individuals (368 T2DM patients and 259 healthy control 
      subjects) part of a well-characterized cohort followed in Riyadh, Kingdom of 
      Saudi Arabia. Genomic DNA was genotyped for the VDR gene single nucleotide 
      polymorphisms of Fok-1, Taq-1, ApaI, and Bsm-I. Analyses were run by allelic 
      discrimination real-time PCR. HLA genotyping was performed as well by PCR using 
      sequence-specific primers, whereas cytokine production was evaluated by FACS. 
      Results showed T2DM to be significantly associated with the VDR Taq1 
      (rs731236-AG) and Bsm-I (rs1544410-CT) genotypes, and the VDR rs1544410-T allele. 
      Cosegregations resulting in significant increases of T2DM odds ratio were 
      detected between Taq1 and Bsm-I VDR polymorphisms and HLA DRB1*04. Notably, the 
      VDR polymorphisms observed to be more frequent in T2DM patients correlated with 
      increased VDR expression and IL-12 production, as well as with metabolic 
      parameters of susceptibility to T2DM, including serum cholesterol and 
      high-density lipoprotein levels. VDR polymorphisms are present in T2DM, and 
      correlate with HLA DRB1*04 and with immunologic and metabolic parameters; results 
      from this study add T2DM to the list of diseases that are likely modulated by an 
      HLA/VDR interaction.
FAU - Al-Daghri, Nasser M
AU  - Al-Daghri NM
AD  - Biomarkers Research Program, Biochemistry Department, College of Science, King 
      Saud University, Riyadh 11451, Kingdom of Saudi Arabia. aldaghri2011@gmail.com
FAU - Al-Attas, Omar
AU  - Al-Attas O
FAU - Alokail, Majed S
AU  - Alokail MS
FAU - Alkharfy, Khalid M
AU  - Alkharfy KM
FAU - Draz, Hossam M
AU  - Draz HM
FAU - Agliardi, Cristina
AU  - Agliardi C
FAU - Mohammed, Abdul Khader
AU  - Mohammed AK
FAU - Guerini, Franca R
AU  - Guerini FR
FAU - Clerici, Mario
AU  - Clerici M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120104
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (Receptors, Calcitriol)
SB  - IM
MH  - Case-Control Studies
MH  - Chromosome Segregation
MH  - Diabetes Mellitus, Type 2/epidemiology/*genetics
MH  - Disease Susceptibility
MH  - Genetics, Population
MH  - HLA-DRB1 Chains/*genetics
MH  - Humans
MH  - *Polymorphism, Genetic
MH  - Receptors, Calcitriol/*genetics
MH  - Saudi Arabia/epidemiology
EDAT- 2012/01/06 06:00
MHDA- 2012/06/12 06:00
CRDT- 2012/01/06 06:00
PHST- 2012/01/06 06:00 [entrez]
PHST- 2012/01/06 06:00 [pubmed]
PHST- 2012/06/12 06:00 [medline]
AID - jimmunol.1101954 [pii]
AID - 10.4049/jimmunol.1101954 [doi]
PST - ppublish
SO  - J Immunol. 2012 Feb 1;188(3):1325-32. doi: 10.4049/jimmunol.1101954. Epub 2012 
      Jan 4.