PMID- 22219691
OWN - NLM
STAT- MEDLINE
DCOM- 20120717
LR  - 20211021
IS  - 1424-8220 (Electronic)
IS  - 1424-8220 (Linking)
VI  - 10
IP  - 6
DP  - 2010
TI  - Development of a quartz crystal microbalance biosensor with aptamers as 
      bio-recognition element.
PG  - 5859-71
LID - 10.3390/s100605859 [doi]
AB  - The ultimate goal in any biosensor development project is its use for actual 
      sample detection. Recently, there has been an interest in biosensors with 
      aptamers as bio-recognition elements, but reported examples all deal with 
      standards, not human serum. In order to verify the differences of aptamer-based 
      biosensor and antibody-based biosensor in clinical detection, a comparison of the 
      performance of aptamer-based and antibody-based quartz crystal microbalance (QCM) 
      biosensors for the detection of immunoglobulin E (IgE) in human serum was carried 
      out. Aptamers (or antibodies) specific to IgE were immobilized on the gold 
      surface of a quartz crystal. The frequency shifts of the QCM were measured. The 
      linear range with the antibody (10-240 mug/L) compared to that of the aptamer 
      (2.5-200 mug/L), but a lower detection limit could be observed in the 
      aptamer-based biosensor. The reproducibility of the two biosensors was 
      comparable. The aptamers were equivalent or superior to antibodies in terms of 
      specificity and sensitivity. In addition, the aptamer receptors could tolerate 
      repeated affine layer regeneration after ligand binding and recycling of the 
      biosensor with little loss of sensitivity. When stored for three weeks, the 
      frequency shifts of the aptamer-coated crystals were all greater than 90% of 
      those on the response at the first day.
FAU - Yao, Chunyan
AU  - Yao C
AD  - Department of Laboratory Medicine, Southwest Hospital, Third Military Medical 
      University, Chongqing 400038, China. yao_yao24@yahoo.com
FAU - Zhu, Tangyou
AU  - Zhu T
FAU - Qi, Yongzhi
AU  - Qi Y
FAU - Zhao, Yuhui
AU  - Zhao Y
FAU - Xia, Han
AU  - Xia H
FAU - Fu, Weiling
AU  - Fu W
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100609
PL  - Switzerland
TA  - Sensors (Basel)
JT  - Sensors (Basel, Switzerland)
JID - 101204366
RN  - 0 (Aptamers, Nucleotide)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Antibody Specificity
MH  - Aptamers, Nucleotide/*chemistry/metabolism
MH  - Biosensing Techniques/*instrumentation
MH  - Blood Chemical Analysis/instrumentation/methods
MH  - *Equipment Design
MH  - Humans
MH  - Immunoglobulin E/analysis/metabolism
MH  - Osmolar Concentration
MH  - Quartz Crystal Microbalance Techniques/*instrumentation
MH  - Substrate Specificity
PMC - PMC3247736
OTO - NOTNLM
OT  - IgE
OT  - antibody
OT  - aptamer
OT  - biosensor
OT  - quartz crystal microbalance
EDAT- 2010/01/01 00:00
MHDA- 2012/07/18 06:00
PMCR- 2010/01/01
CRDT- 2012/01/06 06:00
PHST- 2010/03/30 00:00 [received]
PHST- 2010/04/20 00:00 [revised]
PHST- 2010/05/15 00:00 [accepted]
PHST- 2012/01/06 06:00 [entrez]
PHST- 2010/01/01 00:00 [pubmed]
PHST- 2012/07/18 06:00 [medline]
PHST- 2010/01/01 00:00 [pmc-release]
AID - s100605859 [pii]
AID - sensors-10-05859-v2 [pii]
AID - 10.3390/s100605859 [doi]
PST - ppublish
SO  - Sensors (Basel). 2010;10(6):5859-71. doi: 10.3390/s100605859. Epub 2010 Jun 9.