PMID- 22220194
OWN - NLM
STAT- MEDLINE
DCOM- 20120425
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 6
IP  - 12
DP  - 2011
TI  - Porcine FcgammaRIIb mediates enhancement of porcine reproductive and respiratory 
      syndrome virus (PRRSV) infection.
PG  - e28721
LID - 10.1371/journal.pone.0028721 [doi]
LID - e28721
AB  - Antibody-dependent enhancement (ADE) of virus infection caused by the uptake of 
      virus-antibody complexes by FcgammaRs is a significant obstacle to the development of 
      effective vaccines to control certain human and animal viral diseases. The 
      activation FcgammaRs, including FcgammaRI and FcgammaRIIa have been shown to mediate ADE 
      infection of virus. In the present paper, we showed that pocine FcgammaRIIb, an 
      inhibitory FcgammaR, mediates ADE of PRRSV infection. Stable Marc-145 cell lines 
      expressing poFcgammaRIIb (Marc-poFcgammaRII) were established. The relative yield of 
      progeny virus was significantly increased in the presence of sub-neutralization 
      anti-PRRSV antibody. The Fab fragment and normal porcine sera had no effect. 
      Anti-poFcgammaRII antibody inhibited the enhancement of infection when cells were 
      infected in the presence of anti-PRRSV antibody, but not when cells were infected 
      in the absence of antibody. These results indicate that enhancement of infection 
      in these cells by anti-PRRSV virus antibody is FcgammaRII-mediated. Identification of 
      the inhibitory FcgammaR mediating ADE infection should expand our understanding of 
      the mechanisms of pathogenesis for a broad range of infectious diseases and may 
      open many approaches for improvements to the treatment and prevention of such 
      diseases.
FAU - Qiao, Songlin
AU  - Qiao S
AD  - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan 
      Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural 
      Sciences, Zhengzhou, China.
FAU - Jiang, Zhizheng
AU  - Jiang Z
FAU - Tian, Xiaohui
AU  - Tian X
FAU - Wang, Rui
AU  - Wang R
FAU - Xing, Guangxu
AU  - Xing G
FAU - Wan, Bo
AU  - Wan B
FAU - Bao, Dengke
AU  - Bao D
FAU - Liu, Yonghui
AU  - Liu Y
FAU - Hao, Huifang
AU  - Hao H
FAU - Guo, Junqing
AU  - Guo J
FAU - Zhang, Gaiping
AU  - Zhang G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111229
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antibodies, Viral)
RN  - 0 (Fc gamma receptor IIB)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Receptors, IgG)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/immunology
MH  - Antibody-Dependent Enhancement/*immunology
MH  - Cell Line
MH  - Disease Susceptibility
MH  - Flow Cytometry
MH  - Humans
MH  - Immunoglobulin G
MH  - Kinetics
MH  - Macrophages, Alveolar/virology
MH  - Porcine Reproductive and Respiratory Syndrome/virology
MH  - Porcine respiratory and reproductive syndrome virus/growth & 
      development/*immunology/physiology
MH  - Protein Binding
MH  - Receptors, IgG/*immunology
MH  - Sus scrofa/*immunology/*virology
MH  - Swine/immunology/virology
MH  - Virus Replication/physiology
PMC - PMC3248417
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/01/06 06:00
MHDA- 2012/04/26 06:00
PMCR- 2011/12/29
CRDT- 2012/01/06 06:00
PHST- 2011/08/11 00:00 [received]
PHST- 2011/11/14 00:00 [accepted]
PHST- 2012/01/06 06:00 [entrez]
PHST- 2012/01/06 06:00 [pubmed]
PHST- 2012/04/26 06:00 [medline]
PHST- 2011/12/29 00:00 [pmc-release]
AID - PONE-D-11-15774 [pii]
AID - 10.1371/journal.pone.0028721 [doi]
PST - ppublish
SO  - PLoS One. 2011;6(12):e28721. doi: 10.1371/journal.pone.0028721. Epub 2011 Dec 29.