PMID- 2222049
OWN - NLM
STAT- MEDLINE
DCOM- 19901121
LR  - 20190628
IS  - 0003-4975 (Print)
IS  - 0003-4975 (Linking)
VI  - 50
IP  - 4
DP  - 1990 Oct
TI  - Complement activation and lung permeability during cardiopulmonary bypass.
PG  - 597-601
AB  - Pulmonary dysfunction after cardiopulmonary bypass has been attributed to the 
      damaging effects of complement activation on the lung. To further explore this 
      phenomenon, we measured plasma levels of activated complement components 
      (radioimmunoassay), assessed neutrophil n-formyl-methionyl-leucyl-phenylalanine 
      (FMLP) receptor status (radioligand saturation binding assay), and quantified 
      pulmonary epithelial permeability as radioaerosol lung clearance of technetium 
      99m-labeled diethylenetriamine pentaacetic acid in a series of 8 patients 
      undergoing cardiopulmonary bypass. Significant elevations of plasma C3adesArg, 
      C4adesArg, and C5adesArg levels were seen just after CPB, indicating activation 
      of both the classic and alternate complement pathways. Neutrophil activation was 
      evident as increased expression of neutrophil FMLP surface receptors after 
      bypass. Despite the presence of complement and neutrophil activation, increased 
      pulmonary epithelial permeability was not seen. These data support the hypothesis 
      that complement and neutrophil activation during cardiopulmonary bypass is not 
      associated with acute lung injury, at least not pulmonary epithelial injury. One 
      can therefore infer that increased pulmonary epithelial permeability in patients 
      at high risk for and experiencing sepsis-induced and trauma-induced adult 
      respiratory distress syndrome may be due to factors other than complement and 
      neutrophil activation.
FAU - Tennenberg, S D
AU  - Tennenberg SD
AD  - Department of Surgery, University of Cincinnati College of Medicine, Ohio.
FAU - Clardy, C W
AU  - Clardy CW
FAU - Bailey, W W
AU  - Bailey WW
FAU - Solomkin, J S
AU  - Solomkin JS
LA  - eng
GR  - R01 GM31754/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Ann Thorac Surg
JT  - The Annals of thoracic surgery
JID - 15030100R
RN  - 0 (Receptors, Formyl Peptide)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (complement C3a, des-Arg-(77)-)
RN  - 0 (complement C4a, des-Arg)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 80295-42-7 (Complement C3a)
RN  - 80295-49-4 (Complement C4a)
RN  - VW78417PU1 (Technetium Tc 99m Pentetate)
SB  - IM
CIN - Ann Thorac Surg. 1991 Apr;51(4):695-6. PMID: 2012441
MH  - Capillary Permeability/*physiology
MH  - *Cardiopulmonary Bypass
MH  - Complement Activation/*immunology
MH  - Complement C3a/analysis
MH  - Complement C4a/analysis
MH  - Female
MH  - Humans
MH  - Lung/*physiology
MH  - Lymphocyte Activation/immunology
MH  - Male
MH  - Middle Aged
MH  - N-Formylmethionine Leucyl-Phenylalanine/immunology
MH  - Neutrophils/immunology
MH  - Radioimmunoassay
MH  - Receptors, Formyl Peptide
MH  - Receptors, Immunologic/analysis
MH  - Technetium Tc 99m Pentetate
EDAT- 1990/10/01 00:00
MHDA- 1990/10/01 00:01
CRDT- 1990/10/01 00:00
PHST- 1990/10/01 00:00 [pubmed]
PHST- 1990/10/01 00:01 [medline]
PHST- 1990/10/01 00:00 [entrez]
AID - 0003-4975(90)90196-D [pii]
AID - 10.1016/0003-4975(90)90196-d [doi]
PST - ppublish
SO  - Ann Thorac Surg. 1990 Oct;50(4):597-601. doi: 10.1016/0003-4975(90)90196-d.