PMID- 22222784
OWN - NLM
STAT- MEDLINE
DCOM- 20120420
LR  - 20220331
IS  - 1534-6080 (Electronic)
IS  - 0041-1337 (Linking)
VI  - 93
IP  - 4
DP  - 2012 Feb 27
TI  - Comparative study on signal transduction in endothelial cells after anti-a/b and 
      human leukocyte antigen antibody reaction: implication of accommodation.
PG  - 390-7
LID - 10.1097/TP.0b013e3182424df3 [doi]
AB  - BACKGROUND: Recent development of immunosuppressive therapy has provided a 
      platform for clinical human leukocyte antigen (HLA)- and ABO-incompatible kidney 
      transplantation. However, the prognosis seems to be different between the two. 
      Accommodation, the condition of no injury even in the presence of antidonor 
      antibody, is one of the key factors for successful transplantation with antidonor 
      antibody. The purpose of this study was to compare signal transduction between 
      anti-A/B and anti-HLA antibody reaction and to elucidate the mechanisms 
      underlying accommodation. METHODS: Blood type A- or B-transferase gene was 
      transfected into human EA.hy926 endothelial cells. After cell sorting, A- or 
      B-expressing cells at high levels were obtained. The effects of anti-HLA and 
      anti-A/B antibody binding on complement-mediated cytotoxicity and signal 
      transduction were examined. RESULTS: Preincubation with anti-HLA antibodies only 
      at low levels (<10% of saturation level) or anti-A/B antibodies at high levels 
      (even at near saturation levels) for 24 hr resulted in resistance to 
      complement-mediated cytotoxicity. Anti-A/B antibody ligation inactivated ERK1/2 
      pathway and increased complement regulatory proteins such as CD55 and CD59, 
      whereas anti-HLA ligation activated PI3K/AKT pathway and increased cytoprotective 
      genes such as hemeoxygenase-1 and ferritin H. CONCLUSION: Complement inhibition 
      by upregulation of CD55 and CD59 through ERK1/2 inactivation might play a 
      substantial role in accommodation after ABO-incompatible transplantation, which 
      could also explain the intriguing finding of C4d deposition in the graft without 
      rejection.
FAU - Iwasaki, Kenta
AU  - Iwasaki K
AD  - Department of Applied Immunology, Nagoya University School of Medicine, Nagoya, 
      Japan.
FAU - Miwa, Yuko
AU  - Miwa Y
FAU - Ogawa, Haruko
AU  - Ogawa H
FAU - Yazaki, Satoko
AU  - Yazaki S
FAU - Iwamoto, Masaki
AU  - Iwamoto M
FAU - Furusawa, Tadashi
AU  - Furusawa T
FAU - Onishi, Akira
AU  - Onishi A
FAU - Kuzuya, Takafumi
AU  - Kuzuya T
FAU - Haneda, Masataka
AU  - Haneda M
FAU - Watarai, Yoshihiko
AU  - Watarai Y
FAU - Uchida, Kazuharu
AU  - Uchida K
FAU - Kobayashi, Takaaki
AU  - Kobayashi T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (ABO Blood-Group System)
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (CD55 Antigens)
RN  - 0 (CD59 Antigens)
RN  - 0 (HLA Antigens)
RN  - 9007-36-7 (Complement System Proteins)
RN  - 9013-31-4 (Apoferritins)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Oncogene Protein v-akt)
SB  - IM
CIN - Transplantation. 2012 Feb 27;93(4):354-5. PMID: 22277981
MH  - ABO Blood-Group System/*immunology
MH  - Antibodies, Anti-Idiotypic/*immunology/pharmacology
MH  - Apoferritins/physiology
MH  - CD55 Antigens/physiology
MH  - CD59 Antigens/physiology
MH  - Cells, Cultured
MH  - Complement System Proteins/physiology
MH  - Endothelial Cells/drug effects/immunology/*physiology
MH  - Graft Rejection/immunology/*prevention & control
MH  - HLA Antigens/*immunology
MH  - Heme Oxygenase-1/physiology
MH  - Humans
MH  - MAP Kinase Signaling System/physiology
MH  - Oncogene Protein v-akt/physiology
MH  - Organ Transplantation/*physiology
MH  - Phosphatidylinositol 3-Kinases/physiology
MH  - Signal Transduction/immunology/*physiology
EDAT- 2012/01/10 06:00
MHDA- 2012/04/21 06:00
CRDT- 2012/01/07 06:00
PHST- 2012/01/07 06:00 [entrez]
PHST- 2012/01/10 06:00 [pubmed]
PHST- 2012/04/21 06:00 [medline]
AID - 10.1097/TP.0b013e3182424df3 [doi]
PST - ppublish
SO  - Transplantation. 2012 Feb 27;93(4):390-7. doi: 10.1097/TP.0b013e3182424df3.