PMID- 22223171
OWN - NLM
STAT- MEDLINE
DCOM- 20130708
LR  - 20120320
IS  - 0027-5514 (Print)
IS  - 0027-5514 (Linking)
VI  - 104
IP  - 2
DP  - 2012 Mar-Apr
TI  - A new reducing polyketide synthase gene from the lichen-forming fungus Cladonia 
      metacorallifera.
PG  - 362-70
LID - 10.3852/11-001 [doi]
AB  - Lichens produce unique polyketide secondary metabolites including depsides, 
      depsidones, dibenzofurans and depsones. The biosynthesis of these compounds is 
      governed by polyketide synthase (PKS), but the mechanism via which they are 
      produced has remained unclear until now. We reported the 6-methylsalicylic acid 
      synthase (6-MSAS) type of PKS gene, which is a member of the fungal-reducing 
      PKSs. A cultured mycobiont of Cladonia metacorallifera was employed in the 
      isolation and characterization of a polyketide synthase gene (CmPKS1). The 
      complete sequence information for CmPKS1 was acquired via the screening of a 
      Fosmid genomic library with a 456 bp fragment corresponding to part of the acyl 
      transferase (AT) domain as a probe. CmPKS1 contains beta-ketoacyl synthase (KS), AT, 
      dehydratase (DH), ketoreductase (KR) and phosphopantetheine attachment site (PP) 
      domains.: The domain organization of CmPKS1 (KS-AT-DH-KR-PP) is a typical 
      6-MSAS-type PKS, and the results of phylogenetic analysis showed that CmPKS1 
      grouped with other fungal-reducing PKSs. Quantitative real time PCR analyses 
      showed that CmPKS1 was expressed preferentially in the early growth stage of the 
      axenically cultured mycobiont. Furthermore CmPKS1 expression was found to be 
      dependent on the carbon sources and concentrations in the medium.
FAU - Kim, Jung A
AU  - Kim JA
AD  - Korean Lichen Research Institute, Sunchon National University, Sunchon, Korea.
FAU - Hong, Soon Gyu
AU  - Hong SG
FAU - Cheong, Yong Hwa
AU  - Cheong YH
FAU - Koh, Young Jin
AU  - Koh YJ
FAU - Hur, Jae-Seoun
AU  - Hur JS
LA  - eng
SI  - GENBANK/JF710651
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120105
PL  - England
TA  - Mycologia
JT  - Mycologia
JID - 0400764
RN  - 0 (Fungal Proteins)
RN  - 0 (Polyketides)
RN  - 79956-01-7 (Polyketide Synthases)
RN  - EC 2.3.- (Acyltransferases)
SB  - IM
MH  - Acyltransferases/genetics
MH  - Amino Acid Sequence
MH  - Ascomycota/classification/*enzymology/genetics/isolation & purification
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Fungal Proteins/genetics
MH  - Gene Expression Regulation, Enzymologic
MH  - Genomic Library
MH  - Lichens/classification/*enzymology/genetics/isolation & purification
MH  - Molecular Sequence Data
MH  - Nucleic Acid Hybridization
MH  - Oxidation-Reduction
MH  - Phylogeny
MH  - Polyketide Synthases/*genetics/metabolism
MH  - Polyketides/*metabolism
MH  - Protein Structure, Tertiary
MH  - Real-Time Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
EDAT- 2012/01/10 06:00
MHDA- 2013/07/09 06:00
CRDT- 2012/01/07 06:00
PHST- 2012/01/07 06:00 [entrez]
PHST- 2012/01/10 06:00 [pubmed]
PHST- 2013/07/09 06:00 [medline]
AID - 11-001 [pii]
AID - 10.3852/11-001 [doi]
PST - ppublish
SO  - Mycologia. 2012 Mar-Apr;104(2):362-70. doi: 10.3852/11-001. Epub 2012 Jan 5.