PMID- 22223428
OWN - NLM
STAT- MEDLINE
DCOM- 20120413
LR  - 20131121
IS  - 1524-4539 (Electronic)
IS  - 0009-7322 (Linking)
VI  - 125
IP  - 5
DP  - 2012 Feb 7
TI  - The bispecific SDF1-GPVI fusion protein preserves myocardial function after 
      transient ischemia in mice.
PG  - 685-96
LID - 10.1161/CIRCULATIONAHA.111.070508 [doi]
AB  - BACKGROUND: CXCR4-positive bone marrow cells (BMCs) are critically involved in 
      cardiac repair mechanisms contributing to preserved cardiac function. Stromal 
      cell-derived factor-1 (SDF-1) is the most prominent BMC homing factor known to 
      augment BMC engraftment, which is a limiting step of stem cell-based therapy. 
      After myocardial infarction, SDF-1 expression is rapidly upregulated and promotes 
      myocardial repair. METHODS AND RESULTS: We have established a bifunctional 
      protein consisting of an SDF-1 domain and a glycoprotein VI (GPVI) domain with 
      high binding affinity to the SDF-1 receptor CXCR4 and extracellular matrix 
      proteins that become exposed after tissue injury. SDF1-GPVI triggers chemotaxis 
      of CXCR4-positive cells, preserves cell survival, enhances endothelial 
      differentiation of BMCs in vitro, and reveals proangiogenic effects in ovo. In a 
      mouse model of myocardial infarction, administration of the bifunctional protein 
      leads to enhanced recruitment of BMCs, increases capillary density, reduces 
      infarct size, and preserves cardiac function. CONCLUSIONS: These results indicate 
      that administration of SDF1-GPVI may be a promising strategy to treat myocardial 
      infarction to promote myocardial repair and to preserve cardiac function.
FAU - Ziegler, Melanie
AU  - Ziegler M
AD  - Innere Medizin III, Eberhard Karls Universitat, Tubingen, Otfried-Muller-Strasse 
      10, Tubingen, Germany.
FAU - Elvers, Margitta
AU  - Elvers M
FAU - Baumer, Yvonne
AU  - Baumer Y
FAU - Leder, Christoph
AU  - Leder C
FAU - Ochmann, Carmen
AU  - Ochmann C
FAU - Schonberger, Tanja
AU  - Schonberger T
FAU - Jurgens, Tobias
AU  - Jurgens T
FAU - Geisler, Tobias
AU  - Geisler T
FAU - Schlosshauer, Burkhard
AU  - Schlosshauer B
FAU - Lunov, Oleg
AU  - Lunov O
FAU - Engelhardt, Stefan
AU  - Engelhardt S
FAU - Simmet, Thomas
AU  - Simmet T
FAU - Gawaz, Meinrad
AU  - Gawaz M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120105
PL  - United States
TA  - Circulation
JT  - Circulation
JID - 0147763
RN  - 0 (CXCR4 protein, mouse)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Cxcl12 protein, mouse)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Receptors, CXCR4)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (platelet membrane glycoprotein VI)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cell Survival/drug effects
MH  - *Cell- and Tissue-Based Therapy
MH  - Chemokine CXCL12/metabolism/*pharmacology/therapeutic use
MH  - Collagen/metabolism
MH  - Heart/*drug effects/*physiopathology
MH  - Ischemic Attack, Transient/pathology/physiopathology/*therapy
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Models, Animal
MH  - Myocardial Infarction/pathology/physiopathology/*therapy
MH  - Myocytes, Cardiac/drug effects/pathology
MH  - Neovascularization, Physiologic/drug effects
MH  - Platelet Membrane Glycoproteins/metabolism/*pharmacology/therapeutic use
MH  - Protein Binding
MH  - Receptors, CXCR4/metabolism
MH  - Recombinant Fusion Proteins/*pharmacology
MH  - Treatment Outcome
EDAT- 2012/01/10 06:00
MHDA- 2012/04/14 06:00
CRDT- 2012/01/07 06:00
PHST- 2012/01/07 06:00 [entrez]
PHST- 2012/01/10 06:00 [pubmed]
PHST- 2012/04/14 06:00 [medline]
AID - CIRCULATIONAHA.111.070508 [pii]
AID - 10.1161/CIRCULATIONAHA.111.070508 [doi]
PST - ppublish
SO  - Circulation. 2012 Feb 7;125(5):685-96. doi: 10.1161/CIRCULATIONAHA.111.070508. 
      Epub 2012 Jan 5.