PMID- 22224586
OWN - NLM
STAT- MEDLINE
DCOM- 20120419
LR  - 20161125
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 60
IP  - 4
DP  - 2012 Feb 1
TI  - Simultaneous quantitation of 2-acetyl-4-tetrahydroxybutylimidazole, 2- and 
      4-methylimidazoles, and 5-hydroxymethylfurfural in beverages by 
      ultrahigh-performance liquid chromatography-tandem mass spectrometry.
PG  - 917-21
LID - 10.1021/jf204476x [doi]
AB  - An ultrahigh-performance liquid chromatography (UHPLC) tandem mass spectrometric 
      (MS/MS) method was developed for the simultaneous quantification of 
      2-acetyl-4-tetrahydroxybutylimidazole (THI), 2- and 4-methylimidazoles (2-MI and 
      4-MI), and 5-hydroxymethylfurfural (HMF) in beverage samples. A C30 
      reversed-phase column was used in this method, providing sufficient retention and 
      total resolution for all targeted analytes, with an MS/MS instrument operated in 
      selected reaction monitoring (SRM) mode for sensitive and selective detection 
      using isotope-labeled 4-methyl-d(3)-imidazole (4-MI-d(3)) as the internal 
      standard (IS). This method demonstrates lower limit of quantification (LLOQ) at 1 
      ng/mL and coefficient of determination (r(2)) >0.999 for each analyte with a 
      calibration range established from 1 to 500 ng/mL. This method also demonstrates 
      excellent quantification accuracy (84.6-105% at 5 ng/mL, n = 7), precision (RSD < 
      7% at 5 ng/mL, n = 7), and recovery (88.8-99.5% at 10, 100, and 200 ng/mL, n = 
      3). Seventeen carbonated beverage samples were tested (n = 2) in this study 
      including 13 dark-colored beverage samples with different flavors and varieties 
      and 4 light-colored beverage samples. Three target analytes were quantified in 
      these samples with concentrations in the range from 284 to 644 ng/mL for 4-MI and 
      from 706 to 4940 ng/mL for HMF. THI was detected in only one sample at 6.35 
      ng/mL.
FAU - Wang, Jinyuan
AU  - Wang J
AD  - Thermo Fisher Scientific, 1214 Oakmead Parkway, Sunnyvale, California 94086, 
      United States. leo.wang@thermofisher.com
FAU - Schnute, William C
AU  - Schnute WC
LA  - eng
PT  - Journal Article
DEP - 20120118
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Food Coloring Agents)
RN  - 0 (Imidazoles)
RN  - 70ETD81LF0 (5-hydroxymethylfurfural)
RN  - 94944-70-4 (2-acetyl-4(5)-tetrahydroxybutylimidazole)
RN  - DJ1HGI319P (Furaldehyde)
RN  - Q64GF9FV4I (4-methylimidazole)
RN  - T0049Z45LZ (2-methylimidazole)
SB  - IM
MH  - Carbonated Beverages/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Food Coloring Agents/analysis
MH  - Food Contamination/*analysis
MH  - Furaldehyde/*analogs & derivatives/analysis
MH  - Imidazoles/*analysis
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2012/01/10 06:00
MHDA- 2012/04/20 06:00
CRDT- 2012/01/10 06:00
PHST- 2012/01/10 06:00 [entrez]
PHST- 2012/01/10 06:00 [pubmed]
PHST- 2012/04/20 06:00 [medline]
AID - 10.1021/jf204476x [doi]
PST - ppublish
SO  - J Agric Food Chem. 2012 Feb 1;60(4):917-21. doi: 10.1021/jf204476x. Epub 2012 Jan 
      18.