PMID- 22224860
OWN - NLM
STAT- MEDLINE
DCOM- 20120911
LR  - 20181201
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 80
IP  - 1
DP  - 2012 Apr
TI  - In situ identification of carboxymethyl cellulose-digesting bacteria in the rumen 
      of cattle fed alfalfa or triticale.
PG  - 159-67
LID - 10.1111/j.1574-6941.2011.01295.x [doi]
AB  - A method was developed and used to arrest and stain reducing sugars (glucose) 
      produced by bacteria with cell-surface-associated carboxymethyl cellulase 
      (CMCase) and endoglucanase activities (CMC bacteria) in the rumen of cows fed 
      alfalfa or triticale. Precipitation of silver oxide on the surface of individual 
      cells was observed using cellulolytic bacterial pure cultures with known CMCase 
      activity and rumen mixed cultures. The CMC bacteria in the liquid and solid 
      fractions of the rumen digesta were identified using fluorescence in situ 
      hybridization (FISH) with currently available and newly designed oligonucleotide 
      probes. The CMC bacteria contributed between 8.2% and 10.1% to the total 
      bacterial cell numbers. Most of the CMC bacteria (75.2-78.5%) could be identified 
      by FISH probing. The known cellulolytic populations Ruminococcus flavefaciens, 
      R. albus, and Fibrobacter succinogenes constituted 44.5-53.1% of the total. Other 
      CMC bacteria identified hybridized with the probe Clo549 (11.2-23.0%) targeting 
      members of an uncharacterized genus in Clostridia, the probe Inc852 (8.9-10.7%) 
      targeting members of the family Incertae Sedis III and unclassified 
      Clostridiales, and the probe But1243 (< 1%) designed against members of genus 
      Butyrivibrio. Different forage feeds had no marked effects on the percentage 
      abundances of these identified CMC bacteria. All appeared to be involved in 
      cellulose degradation in the rumen of cows fed either alfalfa or triticale.
CI  - (c) 2011 Federation of European Microbiological Societies. Published by Blackwell 
      Publishing Ltd. All rights reserved.
FAU - Kong, Yunhong
AU  - Kong Y
AD  - Department of Biological Science and Technology, Kunming University, Kunming, 
      China.
FAU - Xia, Yun
AU  - Xia Y
FAU - Seviour, Robert
AU  - Seviour R
FAU - He, Maolong
AU  - He M
FAU - McAllister, Tim
AU  - McAllister T
FAU - Forster, Robert
AU  - Forster R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120118
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 9004-34-6 (Cellulose)
RN  - EC 3.2.1.4 (Cellulase)
RN  - EC 3.2.1.4 (carboxymethylcellulase)
RN  - K679OBS311 (Carboxymethylcellulose Sodium)
SB  - IM
MH  - Animals
MH  - Bacteria/genetics/growth & development/isolation & purification/*metabolism
MH  - Carboxymethylcellulose Sodium/*metabolism
MH  - Cattle
MH  - Cellulase/metabolism
MH  - Cellulose/metabolism
MH  - *Edible Grain
MH  - Female
MH  - Fibrobacter/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - *Medicago sativa
MH  - Poaceae/metabolism
MH  - Rumen/metabolism/*microbiology
MH  - Ruminococcus/metabolism
EDAT- 2012/01/10 06:00
MHDA- 2012/09/12 06:00
CRDT- 2012/01/10 06:00
PHST- 2011/05/11 00:00 [received]
PHST- 2011/12/11 00:00 [revised]
PHST- 2011/12/12 00:00 [accepted]
PHST- 2012/01/10 06:00 [entrez]
PHST- 2012/01/10 06:00 [pubmed]
PHST- 2012/09/12 06:00 [medline]
AID - 10.1111/j.1574-6941.2011.01295.x [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2012 Apr;80(1):159-67. doi: 
      10.1111/j.1574-6941.2011.01295.x. Epub 2012 Jan 18.