PMID- 22224954
OWN - NLM
STAT- MEDLINE
DCOM- 20120709
LR  - 20120207
IS  - 1520-5827 (Electronic)
IS  - 0743-7463 (Linking)
VI  - 28
IP  - 5
DP  - 2012 Feb 7
TI  - Nanoscale characterization of zein self-assembly.
PG  - 2429-35
LID - 10.1021/la204204j [doi]
AB  - Zein, a major protein of corn, is rich in alpha-helical structure. It has an 
      amphiphilic character and is capable of self-assembly. Zein can self-assemble 
      into various mesostructures that may find applications in food, agricultural, and 
      biomedical engineering. Understanding the mechanism of zein self-assembly at the 
      nanoscale is important for further development of zein structures. In this work, 
      high-resolution transmission electron microscopy (TEM) images revealed nanosize 
      zein stripes, rings, and discs containing a 0.35 nm periodicity, which is 
      characteristic of beta-sheet. TEM images were interpreted in terms of the 
      transformation of original alpha-helices into beta-sheet conformation after 
      evaporation-induced self-assembly (EISA). The presence of beta-sheet was also 
      detected by circular dichroism (CD) spectroscopy. Zein beta-sheets self-assembled 
      into stripes, which curled into rings. Rings formed discs and eventually spheres. 
      The formation of zein nanostructures was believed to be the result of beta-sheet 
      orientation, alignment, and packing.
FAU - Wang, Yi
AU  - Wang Y
AD  - Department of Food Science and Human Nutrition, University of Illinois at 
      Urbana-Champaign, Urbana, Illinois 61801, United States.
FAU - Padua, Graciela W
AU  - Padua GW
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120120
PL  - United States
TA  - Langmuir
JT  - Langmuir : the ACS journal of surfaces and colloids
JID - 9882736
RN  - 9010-66-6 (Zein)
SB  - IM
MH  - Microscopy, Electron, Transmission
MH  - Nanostructures/*chemistry
MH  - Particle Size
MH  - Protein Conformation
MH  - Surface Properties
MH  - Zein/*chemical synthesis/chemistry
EDAT- 2012/01/10 06:00
MHDA- 2012/07/10 06:00
CRDT- 2012/01/10 06:00
PHST- 2012/01/10 06:00 [entrez]
PHST- 2012/01/10 06:00 [pubmed]
PHST- 2012/07/10 06:00 [medline]
AID - 10.1021/la204204j [doi]
PST - ppublish
SO  - Langmuir. 2012 Feb 7;28(5):2429-35. doi: 10.1021/la204204j. Epub 2012 Jan 20.