PMID- 22226542
OWN - NLM
STAT- MEDLINE
DCOM- 20121105
LR  - 20190610
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 157
IP  - 1-2
DP  - 2012 May 25
TI  - Salmonella flagellin enhances mucosal immunity of avian influenza vaccine in 
      chickens.
PG  - 69-77
LID - 10.1016/j.vetmic.2011.12.014 [doi]
AB  - Flagellin, a bioactive Toll-like receptor (TLR) 5 ligand, may trigger the innate 
      immunity that in turn is important for subsequent adaptive immune responses. In 
      the present study, the adjuvant effects of the monomeric and polymeric forms of 
      Salmonella flagellin (mFliC and pFliC, respectively) were examined in 
      specific-pathogen free (SPF) chickens immunized intramuscularly (i.m.) or 
      intranasally (i.n.) with formalin-inactivated avian influenza virus (AIV) H5N2 
      vaccines. Results showed that mFliC cooperating with the 64CpG adjuvant 
      significantly induced influenza-specific antibody titers of plasma IgA in the 
      i.m.-vaccinated animals. The nasal IgA levels in the i.n.-mFliC-coadministrated 
      AIV vaccinated chickens were significantly elevated compared to levels observed 
      in the control group (H5N2 vaccine alone). The pFliC cooperating with the 64CpG 
      adjuvant significantly enhanced cell proliferation of splenocytes in the 
      i.m.-vaccinated animals. TLR3 and TLR5 expressions were activated by flagellin 
      stimulation in vitro and in vivo. These results suggest that flagellin can be 
      used as an adjuvant in an AIV H5N2 vaccine, especially for mucosal immunity.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Chaung, Hso-Chi
AU  - Chaung HC
AD  - College of Veterinary Medicine, National Pingtung University of Science and 
      Technology, Pingtung 912, Taiwan, ROC. hcchaung@mail.npust.edu.tw
FAU - Cheng, Li-Ting
AU  - Cheng LT
FAU - Hung, Li-Hsiang
AU  - Hung LH
FAU - Tsai, Pei-Chun
AU  - Tsai PC
FAU - Skountzou, Ioanna
AU  - Skountzou I
FAU - Wang, Baozhong
AU  - Wang B
FAU - Compans, Richard W
AU  - Compans RW
FAU - Lien, Yi-Yang
AU  - Lien YY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111216
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Influenza Vaccines)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Toll-Like Receptor 3)
RN  - 0 (Toll-Like Receptor 5)
RN  - 0 (Vaccines, Inactivated)
RN  - 12777-81-0 (Flagellin)
SB  - IM
MH  - Adjuvants, Immunologic/*administration & dosage
MH  - Administration, Intranasal
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Cells, Cultured
MH  - Chickens/*immunology
MH  - Flagellin/*immunology
MH  - *Immunity, Mucosal
MH  - Immunoglobulin A/blood
MH  - Influenza A Virus, H5N2 Subtype/immunology
MH  - Influenza Vaccines/*immunology
MH  - Influenza in Birds/immunology/*prevention & control/virology
MH  - Recombinant Proteins/immunology
MH  - Salmonella/immunology
MH  - Specific Pathogen-Free Organisms
MH  - Toll-Like Receptor 3/immunology
MH  - Toll-Like Receptor 5/immunology
MH  - Vaccines, Inactivated/immunology
EDAT- 2012/01/10 06:00
MHDA- 2012/11/06 06:00
CRDT- 2012/01/10 06:00
PHST- 2011/10/20 00:00 [received]
PHST- 2011/11/30 00:00 [revised]
PHST- 2011/12/08 00:00 [accepted]
PHST- 2012/01/10 06:00 [entrez]
PHST- 2012/01/10 06:00 [pubmed]
PHST- 2012/11/06 06:00 [medline]
AID - S0378-1135(11)00654-7 [pii]
AID - 10.1016/j.vetmic.2011.12.014 [doi]
PST - ppublish
SO  - Vet Microbiol. 2012 May 25;157(1-2):69-77. doi: 10.1016/j.vetmic.2011.12.014. 
      Epub 2011 Dec 16.