PMID- 22226907
OWN - NLM
STAT- MEDLINE
DCOM- 20120503
LR  - 20201209
IS  - 1873-281X (Electronic)
IS  - 1472-9792 (Linking)
VI  - 92
IP  - 2
DP  - 2012 Mar
TI  - T cell responses to DosR and Rpf proteins in actively and latently infected 
      individuals from Colombia.
PG  - 148-59
LID - 10.1016/j.tube.2011.12.005 [doi]
AB  - Mycobacterium tuberculosis DosR regulon-encoded proteins elicit strong immune 
      T-cell responses in individuals with latent tuberculosis (LTBI). Also, 
      resuscitation (Rpf) proteins can induce such responses. However, variations in 
      the immunogenicity of the DosR and Rpf proteins have been observed in European 
      and African populations, and no data are published from other geographic areas. 
      In Colombian LTBI and patients with recently diagnosed PTB, we therefore studied 
      the immune response to DosR, Rpf, stress, and nominal antigens from Mtb, in 7-day 
      stimulated cultures. Three DosR (Rv1737c, Rv2029c, Rv2628c) and 2 Rpf (Rv0867 and 
      Rv2389c) antigens were recognized most prominently on the basis of the net IFNgamma 
      production (DosR) or the percentage of responding individuals (Rpf). Results show 
      that the selected DosR antigens induced a higher proportion of CD4-T cells 
      producing IFNgamma from LTBI, compared to pulmonary TB patients (PTB), while there 
      were no differences in the proportion of CD8-T cells. An increased frequency of 
      CD4, but not CD8 T-cells with a CD45RO(+)CD27(+) phenotype was observed in LTBI 
      in response to Rv2029c, Rv0867c, and Rv2389c, compared to PTB. The levels of 
      cytokines and chemokines in the supernatants of stimulated cells, showed that the 
      DosR and Rpf antigens induced higher levels of IFNgamma in cultures from LTBI 
      compared to PTB, although the induced pattern of cytokines and chemokines was 
      also antigen dependent. In summary, our results are consistent with the 
      significant immunogenicity of Mtb DosR and Rpf antigens in LTBI individuals, and 
      confirm and extend previously reported data from other TB affected human 
      populations.
CI  - Copyright (c) 2011. Published by Elsevier Ltd.
FAU - Riano, Felipe
AU  - Riano F
AD  - Grupo de Inmunologia Celular e Inmunogenetica, Instituto de Investigaciones 
      Medicas, Facultad de Medicina, Universidad de Antioquia, Carrera 53 #61-30, 
      Medellin, Colombia.
FAU - Arroyo, Leonar
AU  - Arroyo L
FAU - Paris, Sara
AU  - Paris S
FAU - Rojas, Mauricio
AU  - Rojas M
FAU - Friggen, Annemieke H
AU  - Friggen AH
FAU - van Meijgaarden, Krista E
AU  - van Meijgaarden KE
FAU - Franken, Kees L M C
AU  - Franken KL
FAU - Ottenhoff, Tom H M
AU  - Ottenhoff TH
FAU - Garcia, Luis F
AU  - Garcia LF
FAU - Barrera, Luis F
AU  - Barrera LF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120105
PL  - Scotland
TA  - Tuberculosis (Edinb)
JT  - Tuberculosis (Edinburgh, Scotland)
JID - 100971555
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (DosR protein, Mycobacterium tuberculosis)
RN  - 0 (resuscitation-promoting factor, bacteria)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.7.- (Protein Kinases)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antigens, Bacterial/immunology
MH  - Bacterial Proteins/*immunology
MH  - CD4-CD8 Ratio
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Cells, Cultured
MH  - Chemokines/biosynthesis
MH  - Colombia
MH  - Cytokines/biosynthesis/*immunology
MH  - DNA-Binding Proteins
MH  - Female
MH  - Humans
MH  - Immunologic Memory
MH  - Immunophenotyping
MH  - Interferon-gamma/biosynthesis
MH  - Latent Tuberculosis/*immunology
MH  - Male
MH  - Middle Aged
MH  - Mycobacterium tuberculosis/immunology
MH  - Protein Kinases/*immunology
MH  - T-Lymphocyte Subsets/*immunology
MH  - Tuberculosis, Pulmonary/*immunology
MH  - Young Adult
EDAT- 2012/01/10 06:00
MHDA- 2012/05/04 06:00
CRDT- 2012/01/10 06:00
PHST- 2011/10/06 00:00 [received]
PHST- 2011/12/13 00:00 [revised]
PHST- 2011/12/14 00:00 [accepted]
PHST- 2012/01/10 06:00 [entrez]
PHST- 2012/01/10 06:00 [pubmed]
PHST- 2012/05/04 06:00 [medline]
AID - S1472-9792(11)00240-X [pii]
AID - 10.1016/j.tube.2011.12.005 [doi]
PST - ppublish
SO  - Tuberculosis (Edinb). 2012 Mar;92(2):148-59. doi: 10.1016/j.tube.2011.12.005. 
      Epub 2012 Jan 5.