PMID- 22228494
OWN - NLM
STAT- MEDLINE
DCOM- 20120905
LR  - 20211021
IS  - 1573-7438 (Electronic)
IS  - 0049-4747 (Linking)
VI  - 44
IP  - 5
DP  - 2012 Jun
TI  - Prevalence, molecular fingerprinting and drug resistance profile of 
      enterovirulent Escherichia coli isolates from free-ranging yaks of Tawang 
      district, Arunachal Pradesh, India.
PG  - 1063-72
LID - 10.1007/s11250-011-0041-9 [doi]
AB  - Of 273 samples (rectal swab) collected from free-ranging yaks of Tawang district, 
      Arunachal Pradesh, 42 Shiga toxin-producing Escherichia coli (STEC), six 
      enteropathogenic E. coli (EPEC) and 27 enterotoxigenic E. coli (ETEC) strains 
      were isolated. All the STEC and EPEC strains were further investigated for 
      respective stx variants (for STEC only) and additional putative virulence 
      factors. The 27 ETEC strains were also screened for characteristic enterotoxin 
      gene(s) and colonization factors. Occurrence of ETEC was significantly (p < 0.05) 
      higher in the diarrheic yaks and yaks of less than 1 year of age. Majority of 
      enterovirulent E. coli isolates were resistant to amikacin, azithromycin, 
      chloramphenicol, colistin, doxycycline, furazolidone, nalidixic acid, 
      nitrofurantoin, streptomycin and tetracycline. Dendrogram, constructed with 
      molecular fingerprinting profiles obtained from RAPD (Randomly Amplified 
      Polymorphic DNA) and ERIC (Enterobacterial Repetitive Intergenic Consensus) PCR, 
      placed the isolates in different clusters irrespective of their serotypes, 
      virulence gene and drug resistance pattern. Collectively, the study indicates 
      that yaks, being a potential reservoir of multidrug resistant STEC and EPEC, may 
      represent significant risk to public health in this region. Higher recovery of 
      ETEC isolates from yaks with diarrhea points out that ETEC may be a major 
      determinant for repeated occurrence of diarrhea in yaks.
FAU - Bandyopadhyay, Samiran
AU  - Bandyopadhyay S
AD  - National Research Centre on Yak, ICAR, Dirang, 790101 West Kameng, Arunachal 
      Pradesh, India. samiranvet@gmail.com
FAU - Lodh, Chandan
AU  - Lodh C
FAU - Sarkar, Mihir
AU  - Sarkar M
FAU - Ghosh, Monoj K
AU  - Ghosh MK
FAU - Bera, Asit K
AU  - Bera AK
FAU - Bhattacharyya, Debasish
AU  - Bhattacharyya D
FAU - Mondal, Dipak K
AU  - Mondal DK
FAU - Baruah, Kishore K
AU  - Baruah KK
LA  - eng
PT  - Journal Article
DEP - 20120107
PL  - United States
TA  - Trop Anim Health Prod
JT  - Tropical animal health and production
JID - 1277355
RN  - 0 (DNA, Bacterial)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cattle Diseases/*epidemiology/microbiology
MH  - DNA Fingerprinting/veterinary
MH  - DNA, Bacterial/genetics
MH  - Diarrhea/epidemiology/microbiology/*veterinary
MH  - *Drug Resistance, Multiple, Bacterial
MH  - Enteropathogenic Escherichia coli/classification/*genetics/isolation & 
      purification/pathogenicity
MH  - Enterotoxigenic Escherichia coli/classification/*genetics/isolation & 
      purification/pathogenicity
MH  - Escherichia coli Infections/epidemiology/microbiology/*veterinary
MH  - India
MH  - Phylogeny
MH  - Polymerase Chain Reaction/veterinary
MH  - Prevalence
MH  - Random Amplified Polymorphic DNA Technique/veterinary
MH  - Sequence Analysis, DNA/veterinary
MH  - Shiga-Toxigenic Escherichia coli/classification/*genetics/isolation & 
      purification/pathogenicity
MH  - Virulence Factors/genetics/isolation & purification
EDAT- 2012/01/10 06:00
MHDA- 2012/09/06 06:00
CRDT- 2012/01/10 06:00
PHST- 2011/12/08 00:00 [accepted]
PHST- 2012/01/10 06:00 [entrez]
PHST- 2012/01/10 06:00 [pubmed]
PHST- 2012/09/06 06:00 [medline]
AID - 10.1007/s11250-011-0041-9 [doi]
PST - ppublish
SO  - Trop Anim Health Prod. 2012 Jun;44(5):1063-72. doi: 10.1007/s11250-011-0041-9. 
      Epub 2012 Jan 7.